USING HIGH-RESOLUTION CRYSTAL STRUCTURES TO GUIDE THE ENGINEERING OF REAGENT ENZ

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 试剂酶是生命科学研究领域的无名英雄。从限制性内切酶、连接酶到聚合酶和核酸外切酶，这些酶在所有类型的核酸分析中都发挥着关键作用。在这里，我们建议使用SSRL X射线源获得各种试剂酶的高分辨晶体结构，包括DNA聚合酶、核酸外切酶、逆转录酶及其与DNA和专有核苷酸的络合物。所获得的结构信息将被用来设计这些酶的新的活性部位化学，作为更好的生命科学研究工具。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Reagent enzymes are the unsung heroes in the field of life sciences research. From restriction enzymes, ligases, to polymerases and exonucleaes, these enzymes play critical roles in all types of nucleic acid analysis. Here we propose to use SSRL x-ray source to obtain high-resolution crystal structures of various reagent enzymes including DNA polymerase, exonuclease, reverse transcriptase, and their complexes with DNA and proprietary nucleotides. The structural information obtained will be utilized to design novel active site chemistries of these enzymes as better tools for life sciences research.