XAS OF THE INTERMEDIATE FORMED DURING THE REACTIONS OF P450S AND PEROXYNITRITE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 典型的过氧亚硝酸盐(PN)通过酪氨酸硝化来钝化硫代连接的血红素蛋白。这些硝化蛋白被检测到，并被认为在帕金森？S和阿尔茨海默病？S等疾病中发挥了作用。它们也被证明在神经退行性疾病和心血管疾病中存在着重要的作用。Ullrich及其同事报道，PN与P450、S反应生成P450化合物II(一种Fe(IV)氢氧化物物种，类似于细胞色素P450的反弹中间体)。P450-II？这样形成的不同寻常之处在于，它相对稳定，可以高产生产。P450-PN中间体的产率和稳定性与用更传统的氧化剂(如过氧乙酸或间氯苯酸)生成P450-II时的结果形成鲜明对比。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Typically peroxynitrite (PN) inactivates thiolate-ligated hemes proteins through tyrosine nitration. These nitrated proteins are detected and thought to play a role in diseases such as Parkinson?s and Alzheimer?s. They also have been shown to have a significant presence in neurodegenerative and cardiovascular disorders. Ullrich and coworkers reported that the reaction of PN with P450?s yielded P450 compound II (an Fe(IV)hydroxide species, similar to the rebound intermediate of cytochrome P450). The ?P450-II? thus formed was unusual in that it was relatively stable and could be produced in high yield. The yield and stability of the P450-PN intermediate are in stark contrast to the results obtained when P450-II is generated with more traditional oxidants (e.g. peracetic acid or meta-chloroperbenzoic acid).