TBD+DNA

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 猿猴病毒40（SV 40）T抗原蛋白结合DNA复制起点并启动DNA解旋。 该系统已被用作理解真核DNA复制早期步骤的模型。 SV 40 T抗原的起始结合域的结构已经通过NMR解析，但是对于这种蛋白如何结合DNA还没有模型。 我们已经产生了SV 40复制起点和起点结合结构域之间的复合物的晶体。 该结构将有助于理解T抗原的双六聚体如何位于DNA上，以及起源结合是否有助于六聚体复合物的解旋酶活性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The simian virus 40 (SV40) T-antigen protein binds the origin of DNA replication and initiates DNA unwinding. This system has been used as a model for understanding the early steps in eukaryotic DNA replication. The structure of the origin-binding domain of SV40 T-antigen has been solved by NMR, but there is as yet no model for how this protein binds DNA. We have produced crystals of the complex between the origin of SV40 replication and the origin binding domain. The structure will help understand how the double hexamer of T-antigen sits on the DNA, and whether origin binding contributes to the helicase activity of the hexameric complex.