EXAMINING THE MECHANISM OF ACTION OF RECEPTOR TYROSINE KINASES (RTKS) AND THE CE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 该实验室的主要目标是阐明受体酪氨酸激酶（RTK）的作用机制和它们激活的细胞信号传导途径，以了解在正常生物条件下或在RTK及其信号传导途径功能障碍引起的疾病中，该细胞信号传导系统是如何调节的。 我们的目标之一是应用X射线晶体学，以获得RTKs的作用机制及其信号通路的关键组分的分子视图。我们实验室目前正在进行的两个结构生物学项目：1. 与磷脂酶-C-γ或FRS 2复合的FGFR 1和FGFR 2激酶结构域的不同活化磷酸化形式的结构分析[SMALL_ALPHA]。 分析蛋白酪氨酸激酶PYK 2的两个调控区FAT和FERM结构域。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The major goal of the laboratory is to elucidate the mechanism of action of receptor tyrosine kinases (RTKs) and the cellular signaling pathways that they activate in order to understand how this cell signaling system is regulated under normal biological conditions or in diseases caused by dysfunction in RTKs and their signaling pathways. One of our aims is to apply X-ray crystallography in order to obtain a molecular view of the mechanism of action of RTKs and key components of their signaling pathways.Two of the structural biology projects currently being pursued in our laboratory:1.) Analysis of the structure of different activated phosphorylated forms of FGFR1 and FGFR2 kinase domains in complex with phospholipase-C-gamma or FRS2[SMALL_ALPHA].2.) Analysis of the FAT and FERM domains, two regulatory regions of the protein tyrosine kinase PYK2.