SPHERICAL VIRUS STRUCTURES

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 MNV是第一个研究小鼠钙病毒结构和中和作用的病毒。 人类杯状病毒是成人流行性胃肠炎的主要原因，因此 造成重大发病率和经济损失。对人类健康很重要，但并不重要 是否有可能有效地接种针对这些病原体的疫苗；以及 接种疫苗的机制尚不明确。因此，缺乏一种具有 广泛的遗传学和免疫学工具，用于分析发病机制和免疫 阻碍了对这些重要病毒的研究。H.W.维珍实验室已经 发现了第一种被他们命名为老鼠诺如病毒1型或 MNV-1。在杯状病毒中是独一无二的，它们已经形成了一只完整的动物和 细胞培养系统。这为杯状病毒的基础研究提供了新的机会 病毒学和免疫学。 具体地说，我们可以通过分析各种结构来解决一些问题 MNV-1： ？哪些是由抗体识别的重要结构决定因素 提供保护？ ·哪些类型的结构变化会导致病毒的减弱？ 衣壳是动态的吗？中和/保护抗体是否通过影响这些 动力学？ 受体在哪里与衣壳结合？决定衣壳的决定因素是什么？ 物种专一性？

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. MNV is the first murine calcivirus structure and neutralization studies. Human caliciviruses are the major cause of epidemic gastroenteritis in adults, and thereby cause significant morbidity and economic loss. Important to human health, it is not known whether it is possible to effectively vaccinate against these agents, and mechanisms of vaccination are undefined. Thus, the lack of a small animal model with extensive genetic and immunologic tools for the analysis of pathogenesis and immunity has held back research on these important viruses. The H.W. Virgin laboratory has discovered the first murine calicivirus that they have designated Mouse Norovirus 1 or MNV-1. Unique amongst the Caliciviruses, they have developed both a whole animal and cell culture system. This affords new opportunities for basic research into calicivirus virology and immunology. Specifically, some of the questions we can address by analyzing the various structures of MNV-1: ¿ What are the important structural determinants that are recognized by antibodies that confer protection? ¿ What types of structural changes result in attenuation of the virus? ¿ Is the capsid dynamic and do neutralizing/protecting antibodies act by affecting these dynamics? ¿ Where does the receptor bind to the capsid and what are the determinants that dictate species specificity?