STRUCTURE OF MCM21 PROTEIN COMPLEX

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 目标是确定发芽酵母动粒的组成结构，这个子项目的重点是连接内部动粒的蛋白质和直接接触有丝分裂纺锤体微管的蛋白质。在2010年，我们研究了所谓的Coma复合体，它是Ctf19、Ome1、Mcm21和Akp1的异位四聚体。Ctf19和Mcm21形成稳定的异二聚体亚复合体。我们从这种杂二聚体的晶体中收集了大约4？分辨率的数据，并确定了一个近似的结构，现在正在用更好的数据进行调整和精炼。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal is to determine the structures of components of the budding-yeast kinetochore, with a focus for this subproject on the proteins that link the inner kinetochore with proteins that make direct contact with mitotic spindle microtubules. During 2010, we studied the so-called COMA complex, a heterotetramer of Ctf19, Ome1, Mcm21, and Akp1. Ctf19 and Mcm21 form a stable heterodimeric subcomplex. We collected data to about 4 ¿ resolution from crystals of this heterodimer and determined an approximate structure, now being adjusted and refined with better data.