STRUCTURAL STUDIES OF SAM RIBOSWITCHES

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 核糖开关是结构化的RNA，其通过在结合特定小分子时改变其构象来在转录或翻译水平上控制基因表达。我们与俄亥俄州州立大学的Tina Henkins博士合作，发表了两种选择性结合S-腺苷甲硫氨酸（SAM）的核糖开关的晶体结构。这些结构提供了宝贵的见解配体识别机制，在这些核糖开关。我们试图使用APS束时间来确定SAM核糖开关在其载脂蛋白形式的结构，并结合到不同的SAM类似物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Riboswitches are structured RNAs that controls gene expression at either transcription or translation level by changing their conformation upon binding of specific small molecules. In collaboration with Dr. Tina Henkins at Ohio State University, we have published crystal structures of two types of riboswitches that selectively binds to S-adenosyl methionine (SAM). These structures provided valuable insights into the ligand-recognition mechanism in these riboswitches. We seek to use APS beam time to determine the structure of SAM riboswitches in their apo forms, and bound to different SAM analogs.