STOP CODON PROJECT

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 非天然氨基酸通过抑制琥珀终止密码子结合在一起，因为它是大肠杆菌基因组中含量较低的终止密码子。因此，很可能假设琥珀终止密码子在基因组的不同区域被抑制，从而导致突变蛋白质。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Unnatural amino acids are incorporated through the suppression of the amber stop codon, as it is the less abundant stop codon in the genome of the E.coli. Thus it is likely to assume that the amber stop codon is supressed in different regions of the genome resulting in mutant proteins.