ROLE OF HEPATOMA UPREGULATED PROTEIN (HURP) IN CHROMOSOME SEGREGATION

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 HURP是新近发现的一种癌蛋白，参与调节有丝分裂和减数分裂中的染色体分离。它被认为是一种在肝癌中上调的Aurora A底物。HURP是调节RanGTP诱导的微管向双极纺锤体转变的多组分复合体的一部分，也在染色体聚集中发挥作用。它是一种微管相关蛋白，参与稳定K-纤维并将其定位到染色体上，冷冻-EM研究表明，在体外，HURP诱导微管蛋白亚基在微管周围形成片状。本研究的目的是利用非洲爪哇卵提取系统鉴定HURP的相互作用因子，并研究其影响有丝分裂和减数分裂的机制。了解它在染色体聚集中的作用是很重要的，因为染色体的错误分离可能会导致染色体不稳定，而染色体不稳定与癌症密切相关。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. HURP (hepatoma upregulated protein) is a recently discovered oncoprotein, involved in regulation of chromosome segregation in both mitosis and meiosis. It has been identified as an Aurora A substrate upregulated in hepatomas. HURP is a part of a large multi-component complex regulating the transition of RanGTP induced microtubule asters to bipolar spindles, and also have a role in chromosome congression. It is a microtubule associated protein, involved in stabilizing and targeting K-fibers to the chromosomes and cryo-EM studies revealed that HURP induces tubulin subunits to form a sheet around microtubules in vitro. The purpose of this research is to identify HURP interactors and characterize the mechanism by which it influences mitosis and meiosis using Xenopus laevis egg extract system. Understanding of its role in chromosome congression is important as chromosome missegregation may lead to chromosome instability, that have been tightly linked to cancer.