STUDY OF THE DOPAMINE D1 AND D2 RECEPTORS LOCALIZATION IN VIVO

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 多巴胺D_1和D_2受体的定位存在很大争议。一些实验室报告说，100%的纹状体神经元同时表达这两种受体，而另一些实验室报告说，只有一小部分神经元表达这两种受体(从5%到20%)。我们的目标是确定这两种受体是否在成年神经元和胚胎神经元中共定位。为此，我们在实验室培育了表达ECFP标记的多巴胺D1R和EYFP标记的多巴胺D2R的敲击小鼠。这些小鼠将被用来观察正常情况下和滥用药物治疗后内源性受体的定位。此外，我们已经将D1R-ECFP与D2R-EYFP小鼠配对，以便能够进行FRET研究，告诉我们这两个受体是否在体内可能相互作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The localization of dopamine D1 and D2 receptors is highly controversial. Some laboratories have reported that 100% of striatal neurons co-express the two receptors while others have reported that only a fraction of them does (from 5 to 20%. Our goal is to determine if both receptors co-localize in adult as well as in embryonal neurons. For this, we have generated in our lab knockin mice that express dopamine D1R tagged with ECFP and dopamine D2R tagged with EYFP. These mice will be used to visualize the localization of endogenous receptors under normal conditions and after treatment with drugs of abuse. Furthermore, we have mated D1R-ECFP with D2R-EYFP mice to be able to perform FRET studies that will tell us if these two receptors might interact in vivo.