A research and drug development tool for the real time in vitro study of single c

产品说明：在SBIR第二阶段，PicoCal将开发一种高通量仪器，以真实的时间测量单个受体-配体相互作用水平上的细胞粘附变化。该工具在研究和药物开发中具有重要应用，并将使研究人员能够研究和操纵生物活性化合物的粘附，以促进或抑制生理过程。存在显著的工业和科学需求来检测可由趋化因子或其他高生物活性化合物诱导的变化，以阐明细胞内信号传导机制的不同途径并筛选干扰这些途径的新的关键化合物。这些发现可能有助于开发癌症、动脉硬化和自身免疫性疾病（如类风湿性关节炎）的新疗法。在研究和药物开发中，科学家必须研究数千种化合物，以确定化合物是否激活或抑制粘附。此外，癌症等许多疾病都很复杂，需要测试多种分析物和新的关键化合物，以进行准确的药物开发和治疗。在药物开发中需要新的工具来鉴定抑制或促进粘附-或调节受体亲和力的细微变化的生物活性化合物。该仪器还可以帮助研究人员研究细胞粘附变化的机制，例如肿瘤促进和器官特异性转移。主要的研究和制药应用包括：白细胞和造血干细胞粘附到血管，以及其他细胞（骨髓龛）或细胞外基质（ECM）蛋白，血小板粘附，细菌和微生物粘附（生物污染）。

DESCRIPTION: In this SBIR Phase II PicoCal will develop a high throughput apparatus to measure changes in cell adhesion at the level of individual receptor-ligand interactions in real time. This tool finds critical applications in research and drug development and would allow researchers to study and manipulate adhesion by bioactive compounds to promote or inhibit physiological processes. There is a significant industrial and scientific need to detect changes that can be induced by chemokines or other highly bioactive compounds in order to elucidate the different pathways of signaling mechanisms within a cell and to screen for new key compounds interfering with such pathways. These findings may help to develop new therapies for cancer, arteriosclerosis, and autoimmune diseases, like rheumatoid arthritis. In research and drug development scientists have to study thousands of compounds to determine, if a compound activates or inhibits adhesion. In addition, many diseases such as cancer are complex and require testing multiple analytes and new key compounds for accurate drug development and treatment. There is a need for new tools in drug development to identify bio- active compounds that inhibit or promote adhesion - or modulate subtle changes in receptor affinity. The instrument can also aid researchers in studying the mechanisms of cell adhesion changes such as in tumor promotion and organ-specific metastasis. Key research and pharmaceutical applications include: leukocyte and hematopoietic stem cell adhesion to blood vessels, as well as to other cells (bone marrow niche) or to extracellular matrix (ECM) proteins, platelet adhesion, bacteria and micro-organism adhesion (bio-fouling).