开发用于抗 HIV 药物筛选的 HIV Rev 依赖性双报告细胞-猫眼课题宝

开发用于抗 HIV 药物筛选的 HIV Rev 依赖性双报告细胞

批准号：

8296270

负责人：

YUNTAO WU

金额：

$7.07万

依托单位：

GEORGE MASON UNIVERSITY

依托单位国家：

美国

项目类别：

财政年份：

2011

资助国家：

美国

项目状态：

已结题

起止时间：

2011-07-05 至 2013-06-30

来源：

https://reporter.nih.gov/project-details/8296270

关键词：

AIDS/HIV problem Acquired Immunodeficiency Syndrome Anti-HIV Agents Anti-Retroviral Agents Antibodies Biological Assay CCR5 gene Cell Line Cells Chemicals Dependency Development Exhibits Flow Cytometry Gene Expression Grant HIV HIV Antibodies HIV Infections HIV Long Terminal Repeat Hela Cells Highly Active Antiretroviral Therapy Immune In Vitro Inhibitory Concentration 50 Lactamase Lentivirus Vector Libraries Long Terminal Repeats Luciferases Measures Mediating Methodology Mitogens Multi-Drug Resistance Mutation Noise Pharmaceutical Preparations Preclinical Drug Evaluation Probability Reagent Reporter Reporter Genes Reporting Research Research Methodology Response Elements Screening procedure Signal Transduction Suspension substance Suspensions System Technology Testing Transcriptional Activation Translations United States National Institutes of Health Up-Regulation Ursidae Family Viral Viral Load result Virus base cytokine cytotoxicity drug candidate drug discovery high throughput screening inhibitor/antagonist novel novel therapeutics nucleocytoplasmic transport pandemic disease programs promoter prospective repository tat Protein transcription factor treatment planning

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中文摘要

翻译

描述（由申请人提供）：艾滋病毒/艾滋病是一种全球流行病，影响着4000万人，目前尚无治愈方法。该研究旨在开发一种新的报告细胞系，以促进新型抗hiv药物的发现。这种前瞻性的报告细胞系统将采用依赖rev的慢病毒载体，该载体先前已被证明对HIV感染具有高度特异性。该系统将极大地促进基于细胞的新型药物筛选平台的发展。此外，通过采用两个报告者，该系统可用于多种分析格式，并将适用于抗逆转录病毒化合物的高通量筛选。

英文摘要

DESCRIPTION (provided by applicant): HIV/AIDS is a worldwide pandemic afflicting 40 million people, and currently there is no cure. The proposed research is to develop a new reporter cell line to facilitate the discovery of novel anti-HIV drugs. This prospective reporter cell system will employ a Rev-dependent lentiviral vector, which has been shown previously to be highly specific to HIV infection. This novel system will greatly facilitate the development of a novel cell- based drug screening platform. Additionally, by employing two reporters this system can be used in multiple assay formats and would be amenable to high-throughput screens for antiretroviral compounds.

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