Mechanisms of PSGL-1 restriction of HIV virion infectivity

PSGL-1限制HIV病毒粒子感染性的机制

• 批准号: 9927218
• 负责人: YUNTAO WU
• 金额: $ 38.68万
• 依托单位: GEORGE MASON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9927218
• 关键词: Amino Acids; Antiviral Agents; Binding; Biological Assay; CD4 Positive T Lymphocytes; Cell membrane; Cell surface; Cells; Clathrin; Dimerization; Disease remission; Down-Regulation; E-Selectin; Endothelium; Exclusion; Extracellular Domain; Glycoproteins; HIV; HIV Infections; HIV-1; Immune; Infection; L-Selectin; Leukocytes; Link; Lymphoid Cell; Mediating; Membrane; Mucins; Mutagenesis; Myeloid Cells; N-terminal; Natural Immunity; P-Selectin; P-selectin ligand protein; Pathogenesis; Pathway interactions; Phenotype; Process; Proteins; Receptor Cell; Residual state; Role; Site; Structure; Structure-Activity Relationship; Surface; Testing; Tissues; Ubiquitination; Viral; Viral Physiology; Viral reservoir; Virion; Virus Assembly; base; dimer; extracellular; glycosylation; migration; novel therapeutic intervention; particle; receptor; trafficking; tyrosine O-sulfate; ubiquitin-protein ligase; virus host interaction

Project Summary/Abstract Mechanisms of PSGL-1 restriction of HIV virion infectivity Restriction factors are an important component of host innate immunity. Studying the anti-HIV mechanisms of restriction factors is central to understanding virus-host interaction. These mechanisms may also offer new therapeutic strategies to inactivate viral reservoirs to achieve lasting HIV remission. Recently, we have identified a new HIV restriction factor, PSGL-1 (P-selectin glycoprotein ligand-1), that can inactivate the infectivity of HIV virions released from HIV producing cells. PSGL-1 is a dimeric mucin-like 120-KD glycoprotein that is primarily expressed on the surface of lymphoid and myeloid cells. PSGL-1 binds to P-, L-, and E-selectin, and mediates leukocyte tethering and rolling on endothelium for leukocyte migration into inflamed tissues. PSGL-1 is also an INF-γ-regulated factor involved in Th1-mediated anti-viral activity. Our preliminary mechanistic studies further revealed that PSGL-1 is incorporated into viral particles, which blocks HIV Env incorporation and disables the ability of virions to attach to target CD4 T cells for infection. In addition, we found that PSGL-1 is antagonized by Vpu and Nef through surface down-regulation. Based on these preliminary studies, we hypothesize that: (1) PSGL-1-mediated restriction of HIV infectivity involves its specific domains; (2) PSGL-1 restricts HIV infectivity likely through competitive exclusion of Env incorporation during viral assembly and steric hindrance of Env binding to cell receptors (3) Nef-mediated PSGL-1 down-regulation is likely achieved through linking PSGL-1 to components of clathrin-dependent trafficking pathways. In this application, we will pursue the following aims: Specific Aim 1 is to characterize PSGL-1 for inactivating HIV infectivity. We propose to identify PSGL-1 domains key to restricting HIV-1. We will determine the structure-function relationship of PSGL-1, defining the roles of PSGL-1 dimerization, N- terminal glycosylation and tyrosine sulfation, N-terminal decameric repeats, and the polybasic region in restricting HIV. Specific Aim 2 is to perform mechanistic studies of PSGL-1 inactivation of HIV viral infectivity. We hypothesized that PSGL-1 restricts HIV infectivity likely through two possible mechanisms: (1) competitive exclusion of Env incorporation during viral assembly; (2) steric hindrance of residual Env binding to cell receptors. We will test these two hypotheses to determine the mechanisms of action. Specific Aim 3 is to study the mechanism of Nef-mediated surface down-regulation of PSGL-1. We will identify functional domains of Nef and PSGL-1 for their involvement in PSGL-1 down-regulation. Nef-mediated PSGL-1 downregulation may facilitate viral spread in immune cells.

项目总结/摘要 PSGL-1限制HIV病毒粒子感染性的机制 限制性因子是宿主天然免疫的重要组成部分。研究抗艾滋病毒的机制 限制因子是理解病毒-宿主相互作用的核心。这些机制还可以提供新的 治疗策略，以达到持久的艾滋病毒缓解。最近我们 发现了一种新的HIV限制因子PSGL-1（P-选择素糖蛋白配体-1）， 从HIV产生细胞释放的HIV病毒粒子的感染性。PSGL-1是一种粘蛋白样二聚体，分子量为120-KD 主要在淋巴细胞和骨髓细胞表面表达的糖蛋白。PSGL-1与P-，L-， 和E-选择素，并介导白细胞束缚和滚动内皮细胞迁移到 发炎的组织PSGL-1也是一种INF-γ调节因子，参与Th 1介导的抗病毒活性。我们 初步的机制研究进一步揭示了PSGL-1被整合到病毒颗粒中， HIV Env掺入并使病毒体附着于靶CD 4 T细胞以进行感染的能力丧失。在 此外，我们还发现Vpu和Nef通过下调PSGL-1的表面表达而拮抗PSGL-1。基于 这些初步研究，我们假设：（1）PSGL-1介导的HIV感染性限制涉及其 （2）PSGL-1可能通过竞争性排斥Env来限制HIV感染性 （3）Nef介导的病毒组装和Env与细胞受体结合的空间位阻 PSGL-1下调可能是通过将PSGL-1与网格蛋白依赖性细胞因子的组分连接来实现的。 贩运途径。在本申请中，我们将追求以下目标：具体目标1是表征 PSGL-1用于灭活HIV感染性。我们建议确定PSGL-1域的关键限制HIV-1。我们 将确定PSGL-1的结构-功能关系，定义PSGL-1二聚化、N- 末端糖基化和酪氨酸硫酸化，N-末端十聚体重复序列，以及 限制艾滋病毒。具体目标2是进行PSGL-1灭活HIV病毒的机制研究 传染性我们假设PSGL-1可能通过两种可能的机制限制HIV感染性：（1） 在病毒装配过程中Env掺入的竞争性排斥;（2）残留Env结合的空间位阻 细胞受体。我们将测试这两个假设，以确定作用机制。具体目标3是 研究Nef介导的PSGL-1表面下调的机制。我们将确定功能 Nef和PSGL-1的结构域参与PSGL-1下调。Nef介导的PSGL-1 下调可促进病毒在免疫细胞中的传播。