Fluorescence microscopy Shared Resource

荧光显微镜(FM)共享资源为以下方面提供了广泛的显微镜服务 亨斯迈癌症研究所(HCI)成员和整个犹他大学研究界。这个 共享资源的主要重点是提供最先进的工具、软件和专业知识 对于活细胞和固定细胞的成像是必要的。该设施有两个奥林巴斯FV1000光谱共焦 显微镜，两个FV300共焦，一个FVX双光子共焦，一个Path 855高通量共焦，一个 横河旋转圆盘共焦用于微光活细胞成像，以及两台配备有 数码相机。对图像的3D渲染、量化和去卷积的计算支持(例如 从不提供的速度，位平面的Imaris)也被提供。FM资源提供以下方面的培训 通过直接援助和通过正规大学课程利用现有技术和设备。 调频共享资源为以实验室为基础的科学项目(核控制)成员提供服务 细胞生长和分化；细胞反应和调节；成像、诊断和治疗； 和胃肠癌)，使它们能够可视化细胞的形态、行为和分布 在正常和患病状态下，分子进入细胞室。调频共享资源 加强对基因表达、细胞凋亡和细胞分裂的研究。它可以描述肿瘤生长的特征 和形态。 Chris Rodesch博士在一名工作人员的协助下指导资源。仪器设备和 调频共享资源的工作人员被安置在一个中心位置。 FM共享资源是一个机构管理的设施，受到大学和HCI的监督 领导力。有一个学院咨询委员会，并通过以下方式审查资源以确保用户满意 年度调查。该设施是按服务收费(按存储容量使用计费)运营的。调频共享的使用 癌症中心成员提供的资源与同行评审的资金比例为54%。向以下机构申请资金 CCSG将支付拟议资源预算的15%(44,733美元)。FM共享资源具有 有足够的容量供癌症中心成员额外使用。

The Fluorescence Microscopy (FM) Shared Resource provides a broad range of microscopy services for Huntsman Cancer Institute (HCI) members and the entire University of Utah research community. The primary focus of the Shared Resource is to provide state-of-the-art instrumentation, software, and expertise necessary for the imaging of live and fixed cells. The facility has two Olympus FV1000 spectral confocal microscopes, two FV300 confocals, an FVX 2Photon confocal, a Pathway 855 high-throughput confocal, a Yokagowa spinning disk confocal for low-light live cell imaging, and two widefield microscopes equipped with digital cameras. Computational support for 3D rendering, quantification, and deconvolution of images (e.g.. Velocity from Improvision, Imaris from Bitplane) is also provided. The FM Resource provides training in the use of current techniques and equipment by direct assistance and through formal University courses. The FM Shared Resource serves members of the HCI laboratory-based scientific Programs (Nuclear Control of Cell Growth and Differentiation; Cell Response and Regulation; Imaging, Diagnostics, and Therapeutics; and Gastrointestinal Cancers), enabling them to visualize cell morphology and behavior as well as distribution of molecules into cellular compartments in both normal and diseased states. The FM Shared Resource enhances studies of gene expression, apoptosis, and cell division. It allows characterization of tumor growth and morphology. Chris Rodesch, PhD, directs the Resource with the assistance of one staff member. The instrumentation and staff of the FM Shared Resource are housed in a central location. The FM Shared Resource is an institutionally managed facility with supervision from both University and HCI leadership. There is a Faculty Advisory Committee and the Resource is reviewed for user satisfaction by annual surveys. The facility is operated on a fee-for-service basis (chargeback). Use of the FM Shared Resource by Cancer Center members with peer-reviewed funding is 54 percent. Funds are requested from the CCSG to cover 15 percent ($44,733) of the proposed Resource budget. The FM Shared Resource has ample capacity for additional use by Cancer Center members.