Golgi Studies of Schizophrenia

摘要 高尔基体染色显示了树突的解剖结构。而高尔基方法 已经在精神分裂症中产生了一些最有力的组织学发现，研究表明 使用这些技术并不常见，可能是因为这些技术已经 很难申请，而且出了名的反复无常。我们已经开发出一种新的改进型 高尔基-考克斯方法产生了前所未有的可靠性，具有一致的染色 从70多个连续的尸检案例中找到了100多个街区。有一个 均匀分布的染色神经元，允许系统随机采样 用于分析的神经元。我们建议使用这种材料来研究树枝分枝和 20例精神分裂症患者与40例年龄、性别匹配的脊柱密度 无精神疾病，均通过心理尸检进行临床评估。我们会 分析背侧(BA9)和腹侧(BA45)的树突分支和棘突密度 前额叶皮质，以及海马区(下丘、CA3、CA1和齿状回 回)。由于从积木上切下的每一段都染色得很好，我们建议共享 与希望执行额外分析的其他实验室一起进行幻灯片。 此应用程序还包括我们正在进行的大脑收集的延续 马其顿共和国的标本和心理尸检。这一系列 为我们的高尔基体研究和许多其他的研究提供新鲜的组织 精神疾病的神经生物学，无论是在我们系还是在其他机构。 标本和数据保存在我科，并经过严格的临床， 神经病理学和毒理学综述。

Abstract Golgi staining reveals the anatomical structure of dendrites. While Golgi methods have yielded some of the most robust histological findings in schizophrenia, studies that use these techniques are uncommon, probably because the techniques have been difficult to apply and notoriously capricious. We have developed a new modification of the Golgi-Cox method that has yielded unprecedented reliability, with consistent staining in over 100 consecutive blocks from over 70 consecutive autopsy cases. There is a uniform distribution of stained neurons that allows systematic random sampling of neurons for analysis. We propose to use this material to study dendritic arborization and spine density in 20 schizophrenia subjects matched by age and sex to 40 subjects without psychiatric disease, all evaluated clinically by psychological autopsy. We will analyze dendritic arborization and spine density in dorsal (BA9) and ventral (BA45) prefrontal cortex, and in the hippocampal formation (subiculum, CA3, CA1, and dentate gyrus). Since every section cut from a block is stained well, we propose to share the slides with other laboratories that wish to perform additional analyses. This application also includes continuation of our ongoing collection of brain specimens and psychological autopsies in the Republic of Macedonia. This collection provides the fresh tissue for our Golgi studies and for many other studies of the neurobiology of psychiatric illnesses, both in our department and at other institutions. The specimens and data are stored in our department and subjected to rigorous clinical, neuropathological, and toxicological review.