Flow Cytometry and Cell Sorting Core (Core 3)

摘要 炎症是一个复杂的生物过程，涉及循环免疫细胞以及组织和器官的细胞组成的许多变化。确定这些细胞数量和比例的变化，细胞分化和凋亡的各种状态，以及调节下游通路的细胞因子和信号分子的表达模式，对于研究炎症过程至关重要。这些变化中的许多可以通过流式细胞术和分选以及基于微流控生物技术的各种其他技术来密切监测，例如蛋白质的多重ELISA法和RNA的实时PCR分析。其中许多分析需要昂贵的设备和技术专业知识，这是初级研究人员(如科布雷关于膳食补充剂和炎症的科布雷提案中的塔吉特学院)负担不起的。此外，在使用这些技术时，正确设置操作参数和数据解释通常是困难的，需要有经验的操作员专门从事该技术。流式细胞术和细胞分选核心将设在南卡罗来纳大学医学院现有的仪器研究设施(IRF)中，它将提供完成目标研究人员就Cobre提案提出的这些类型实验所需的所有技术和专业知识。这一核心将由现任流式细胞仪实验室主任监督。将有两名隶属于IRF的教职员工，他们可以在实验设计、设备操作和微流体仪器培训方面提供帮助。除了个人互动，授课课程、研讨会和讲习班通常是通过国际扶轮基金会赞助的。这些资料将提供给目标调查员实验室的成员。

Abstract Inflammation is a complex biological process that involves many changes in the cell composition of circulating immune cells as well as tissues and organs. Identifying these changes in cell number and ratios, the status of various states of cell differentiation and apoptosis, and the expression patterns of cytokines and the signaling molecules that regulate the downstream pathways is essential in studying the process of inflammation. Many of these changes can be closely monitored by flow cytometry and sorting, and various other techniques based on micro-fluidic biotechnology such as multiplex ELISA assays for proteins and real time PCR for RNA. Many of these assays require expensive equipment and technical expertise that is not affordable by junior investigators such as the Target Faculty in this COBRE proposal on Dietary Supplements and Inflammation. In addition, setting operating parameters correctly and data interpretation when using these techniques is often difficult and requires experienced operators dedicated to the technology. The Flow Cytometry and Cell Sorting Core, which will be housed in the established Instrumentation Research Facility (IRF) at the University of South Carolina School of Medicine, will provide all of the technology and expertise required to complete these types of experiments proposed by the Target Investigators on this COBRE proposal. This core will be supervised by the current Director of the Flow Cytometry lab. There will be two faculty members who are affiliated with the IRF available for assistance with experimental design, operation of equipment, and training on micro-fluidics instrumentation. In addition to individual interactions, didactic courses, seminars and workshops are often sponsored through the IRF. These will be made available to members of the Target Investigator laboratories.