Oogonial Stem Cell Characterization in POI and NHP

在这个令人兴奋的新兴领域，在过去的一年里，我们投入了大量时间来建立人类和动物方案以及基础科学技术，以便在我们的实验室开始这一新的研究路线。 为了检验假定的干细胞的潜力，在过去的一年里，我们在实验室开始了一个非人类灵长类卵子模型。目前致力于采集卵巢组织和建立卵原干细胞分离和培养技术。 在人类中，我们的目标是在原发性卵巢功能不全/卵巢早衰、卵巢储备减少和医源性卵巢衰竭的疾病模型中研究这些细胞。在过去的一年里，我们已经启动了一项IRB批准的方案，从患有这些疾病的妇女身上收集卵巢组织。我们将很快开始招募患者参加这项研究。

In this exciting, burgeoning field, over the past year we have invested substantial time in establishing human and animal protocols as well as basic science techniques to begin this new line of investigation in our laboratory. To examine the potential of putative stem cells, over the past year we have started a non-human primate oogonial model in our lab. Current efforts are devoted towards harvesting of ovarian tissue and establishing oogonial stem cell isolation and culture techniques. In humans, our aim is to study these cells in a disease model of primary ovarian insufficiency/ premature ovarian failure, diminished ovarian reserve and iatrogenically induced ovarian failure. Over the past year we have started an IRB approved protocol to collect ovarian tissues from women with these conditions. We will begin enrolling patients for this study shortly.