NPC-16 Patchliner Octo (8 Channels)

描述(申请人提供)：当今离子通道研究的一个重要瓶颈是需要使用手动膜片钳记录来获得高质量的数据。威斯康星大学在药物开发的安全分析、治疗药物作用机制以及遗传性和获得性心律失常的分子基础方面的研究将随着新技术的获得而显著加强，这将显著增加实验的吞吐量。在熟练的手动贴片技术人员手中，每天只能可靠地收集几个数据点，而Nanion Patchliner NPC-16是一种自动膜片钳设备，允许每天收集多达500个数据点，基本上无人值守。功能包括具有稳定访问阻力的巨型记录，实现生理相关条件的温度控制，细胞内外隔室的灌流，与培养细胞、原代细胞和含有纯化蛋白的双层的兼容性，以及使用穿孔贴片避免细胞内成分透析的选项。这些能力中的许多无法使用手动贴片复制(例如，同时应用于细胞外和细胞内隔室)，或者非常困难(在生理温度下贴片)。用户都对包括膜片钳在内的多种电生理学方法有丰富的经验，负责监督日常操作的操作技术人员也是如此。制定了一项管理计划，允许在线登记设备使用情况和主要用户之间的交流。预计该设备不仅将提高NIH资助的五个用户的生产率，实现拨款目标，还将为合作和实验打开机会，这是以前用传统技术无法想象的。

DESCRIPTION (provided by applicant): A significant bottleneck in ion channel research today is the need to use manual patch clamp recording to obtain high quality data. Research at the University of Wisconsin on safety assays for drug development, mechanisms of therapeutic drug action and the molecular basis for inherited and acquired arrhythmias would be significantly enhanced with the acquisition of new technology that dramatically increases experimental throughput. Whereas just a few data points can be reliably collected per day at the hands of a skilled manual patch technician, the Nanion Patchliner NPC-16 is an automated patch clamp device that allows the collection of up to 500 data points per day, largely unattended. Capabilities include gigaseal recordings with stable access resistance, temperature control to achieve physiologically-relevant conditions, perfusion of both intra- and extracellular compartments, compatibility with cultured cells, primary cells and bilayers containing purified proteins, and the option of using perforated patch to avoid dialysis of intracellular components. Many of these capabilities cannot be replicated using the manual patch (e.g., simultaneous application to extra- and intracellular compartments) or are extraordinarily difficult (patching at physiological temperatures). The users all have extensive experience with a wide range of electrophysiology approaches including patch clamp, as does the operating technician who will oversee daily operations. A management plan is developed allowing for online registration for equipment use and communication among primary users. It is expected the device will not only enhance the productivity toward grant aims of the five NIH-funded users, but will open opportunities for collaborations and for experiments not previously conceivable with traditional technologies.