TISSUE CULTURE CORE LABORATORY

组织培养实验室的目标是支持项目的研究人员 他们使用细胞模型，因此，它是当前计划中所有项目的组成部分。此外 为了提供专业知识和培训，组织培养实验室提供必要的设备和专用的 理想地适合并维持用于成功制备细胞培养物的空间。如此专用的空间 项目1和项目2的研究人员无法获得其他设备;此外， 培养实验室配备了大量的细胞，用于项目3中提出的实验， 胆固醇逆向转运的体内测定。实验室提供多种服务，例如标准化 用于细胞培养的方案、材料和试剂;广泛使用的细胞系的现成供应;常规制备 小鼠腹腔巨噬细胞等原代细胞的研究、人员培训、新方法的开发; 细胞系的获得和储存;细胞的瞬时和稳定转染;和蛋白质印迹。因此 中央组织培养核心设施有几个优点：1）它节省空间，消除了需要 为冗余设备，并考虑到成本效益和及时采购用品; 2）它确保 程序和细胞系的标准化; 3）它有助于将新的细胞模型引入到 （4）作为一个培训机构。核心B在本赠款周期的使用范围很广。超过 在过去的一年里，该中心平均每周为以下细胞库提供细胞、试剂和材料： 实验：3个外排实验、1个RCT实验和2个原代小鼠巨噬细胞实验。 该实验室还提供了每周一次的蛋白质印迹检测SR-BI，ABCA 1和最近 ABCG 1.核心B也从冷冻储备中回收细胞供研究人员使用，并提供细胞用于 HDL生物合成研究每月至少2次。我们预计这种使用模式将继续在 项目1- 55%;项目2-15%;项目3 -15%。 3-30 %.

The objective of the tissue culture laboratory is to support the investigators of the Program Project who use cell models and, as such, it is an integral part of all the projects in the current program. In addition to providing expertise and training, the tissue culture laboratory provides essential equipment and dedicated space that is ideally suited and maintained for successful preparation of cell cultures. Such dedicated space and equipment is not otherwise available to investigators in Projects 1 and 2; moreover, only the tissue culture laboratory is equipped to provide large numbers of cells for experiments proposed in project 3 for in vivo assay of reverse cholesterol transport. The laboratory provides numerous services e.g. standardized protocols, materials and reagents for cell culture; a ready supply of widely used cell lines; routine preparation of primary cells such as mouse peritoneal macrophages, personnel training; development of new methods; procurement and storage of cell lines; transient and stable transfection of cells; and Western blots. Thus, a centralized Tissue Culture Core facility has several advantages: 1) it conserves space, eliminates the need for redundant equipment and allows for the cost effective and timely acquisition of supplies; 2) it ensures standardization of procedures and cell lines; 3) it facilitates the introduction of new cell models into the program; 4) serves as a training facility. Core B usage in the present grant cycle has been extensive. Over the past year the core has, on a weekly average, provided cells, reagents and materials for the following cellbased experiments: 3 efflux experiments, 1RCT experiment, and 2 primary mouse macrophage experiments. The laboratory has also provided weekly Western blots for detection of SR-BI, ABCA1 and more recently ABCG1. Core B has also recovered cells from frozen stocks for use by investigators and provided cells for studies of HDL biogenesis at least 2 times per month. We expect this pattern of use to continue in the current cycle and anticipate the following core usage distribution: Project 1- 55%; Project 2-15% and Project 3-30 %.