Genome wide siRNA screen for identifying host factors required for ZAP function

描述（由申请方提供）：披膜病毒科甲病毒属的病毒引起显著的人类和动物发病率和死亡率。目前还没有针对这些病毒引起的疾病的具体治疗方法。锌指抗病毒蛋白（ZAP）是一种宿主蛋白，其表现出对该属中的病毒以及逆转录病毒科和丝状病毒科中的病毒的有效抑制。该项目的目标是使用全基因组siRNA筛选方法来鉴定ZAP功能所需或促进ZAP功能的宿主因子。我们预计，这些结果将有助于全面了解ZAP功能的重要因素。这些信息将为解释ZAP功能的现有信息提供基础，并将激发新的假设驱动的研究方向。最终，这项工作可能会激发新的治疗方法的想法，这可能有助于预防这些病毒引起的毁灭性疾病。

DESCRIPTION (provided by applicant): Viruses in the Alphavirus genus of the Togaviridae family cause significant human and animal morbidity and mortality. There is currently no specific treatment for diseases caused by these viruses. The zinc-finger antiviral protein (ZAP) is a host protein that demonstrates potent inhibition of viruses in this genus, as well as viruses in the Retroviridae and Filoviridae families. The objectives of this project are to use a genome-wide siRNA screening approach to identify host factors that are required for, or facilitate, ZAP function. We anticipate the results will help provide a comprehensive picture of the factors important for ZAP function. This information will provide the basis for interpretation of the curret information regarding ZAP's function and will stimulate new hypothesis-driven research directions. Ultimately, ideas for new approaches to treatment may be stimulated by this work, which may help prevent the devastating diseases caused by these viruses.