Reagent/Service Core

组成该计划项目的三个研究项目都集中在了解导致低级别、非侵袭性和高级别浸润性膀胱癌这两种主要途径的膀胱癌发生的分子和细胞事件和机制。除了将各个项目连接成一个连贯的计划的智力和实验协同作用外，三个拟议的研究计划中的每一个都依赖于一套共同的关键试剂和服务，这些试剂和服务可以持续提供，以确保其成功。这些包括但不限于代表不同阶段膀胱癌的一组培养细胞系、吴学如博士开发的不同独特的膀胱癌小鼠模型、重组蛋白、核酸试剂、抗体和cDNA体。显然，让每个实验室独立地生产、采购、表征和维护所有这些试剂是没有意义的，也不是对时间、金钱和资源的有效利用。相反，我们将建立一个集中的试剂和服务核心，其目的之一是作为关键试剂的中央存储库，并为这些重要的研究工具提供中央供应，这些工具可以由参与该计划项目的所有人共享。除了成本效益外，这种方法还提供了确保这些共享试剂在各个项目中的质量的优势。从一个集中的核心提供细胞系、组织和动物模型，消除了试剂处理的可变性，促进了三个单独项目之间的数据比较。此外，它还促进了PI之间的更多互动，并防止在紧急情况下意外丢失宝贵的试剂，例如停电(我们上一次经历停电是在2003年)。最后，核心还将为个别项目提供病理学和统计支持。在计划项目申请的这一部分，我们描述了这些试剂，核心如何发挥控制质量和维护数据库的作用，核心对单个研究项目的贡献，以及核心服务优先使用的计划。核心中心的具体目标是：(I)保持集中供应和分发一套共同的核心试剂，这对支持个别组成部分项目的研究工作至关重要；(Ii)收集人类和老鼠样本并提供必要的病理学支持服务；以及(Iii)提供专门的统计支持。

The three research projects that comprise this Program Project are all focused on understanding molecular and cellular events and mechanisms leading to bladder carcinogenesis of both major pathways of low-grade, non-invasive and high-grade, invasive bladder cancer. In addition t o t h e intellectual and experimental synergies that link the individual projects into a cohesive program, each ofthe three proposed research plans all depend upon a common set of critical reagents and services made available on a continuous basis for their success. These include but are not limited to a set of cultured cell lines that represent different stages of bladder cancer, different unique mouse models of bladder cancer developed by Dr. Xue-Ru Wu, recombinant proteins, nucleic acid reagents, antibodies and cDNA constructs. Obviously, it would not make sense, nor would It be an efficient use of time, money, and resources for each laboratory to Independently generate, procure, characterize, and maintain all these reagents. Instead, we will establish a centralized Reagent and Service Core, one of whose purposes is to serve as a central repository for key reagents and provide a central supply for these important research tools that can be shared by all involved in this Program Project. In addition to the cost-effectiveness, this approach offers the advantage of ensuring the quality of these shared reagents across the individual projects. Providing supplies of cell lines, tissues and animal models from a centralized core eliminates variability in reagent handling, facilitating data comparison amongst the three individual projects. Furthermore, it promotes additional interactions among the Pis and guards against accidental loss of Invaluable reagents in emergency situations such as power outages (which we last experienced in 2003). Finally, the Core will also provide pathology and statistical support for the individual projects. In this section ofthe Program Project application, we describe these reagents, how the Core functions to control quality and maintain databases, the contribution ofthe core to the individual research projects, and a plan for prioritizing usage of core services. The specific aims ofthe Core are: i) To maintain a centralized supply and distribute a common core set of reagents vital to support the research efforts of individual component projects; ii) To collect human and mouse specimens and provide the necessary pathology support service; and iii) To provide dedicated statistical support.