RGB-286638 as a radiation sensitizer

用多种癌细胞株进行的实验表明，rgb-286638在所有被测细胞系中都是一种有效的辐射增敏剂，包括乳腺癌细胞系、前列腺癌细胞系、神经母细胞瘤细胞系和肺癌细胞系。在多个细胞系中发现了有效的辐射增敏作用，当将该化合物添加到细胞中时，辐射剂量增加了近50%。我们已经证明，这些影响与处理细胞中双链DNA断裂的增加以及这些断裂的修复速度较慢有关。关于死亡机制的实验表明，细胞主要是通过有丝分裂灾难死亡的，这是辐射诱导的细胞死亡的典型现象。Rgb-286638对辐射的影响也在小鼠异种移植瘤模型中进行测试。我们已经开始准备这些结果以供发表。这种化合物在人类身上有很大的应用前景。

Experiments with a number of carcinoma cell lines have shown that RGB-286638 is a potent radiation sensitizer in all cell lines tested, including breast cancer cell lines, prostate cancer cell lines, neuroblastoma cell lines, and lung cancer cell lines. Potent radiation sensitization was seen in multiple cell lines, where a nearly 50% enhancement of radiation dose was achieved when the compound was added to cells. We have demonstrated that these effects correlate with increased presence of double stranded DNA breaks in treated cells, and with slower repair of these breaks. Experiments on the mechanism of death suggest that cells die predominantly by mitotic catastrophe, as is typical for radiation-induced cell death. The effects of RGB-286638 with radiation are also being tested in a mouse xenograft tumor model. We have begun preparing these results for publication. This compound holds great promise for use in humans.