A novel pre-defined CDR library for selection of affinity reagents

我们打算改进亲和试剂发现过程，使其不仅比目前可能的更便宜和更快，而且还使我们生产的试剂比目前的单克隆抗体更通用和有用。我们提出利用单链可变片段（scFv）抗体的预定义合成互补决定区（cdr）进行体外组合重组，合成一种新型的高度多样化的合成展示文库。我们将这种新的库类型称为“预定义CDR”（PDC）库。这种新颖的文库系统将具有的特性，也将使它能够极大地促进下游亲和试剂的选择和成熟过程。讨论了利用微流控技术和乳化液筛选技术检测该文库的方法。

DESCRIPTION: We intend to refine the affinity-reagent discovery process so that it is not only cheaper and faster than currently possible, but also so that we produce reagents that are more versatile and useful than current monoclonal antibodies. We propose to use in vitro combinatorial recombination of pre-defined synthetic complementarity determining regions (CDRs) of single chain variable fragment (scFv) antibodies to synthesize a novel type of highly diverse synthetic display library. We have termed this new library-type as a 'pre-defined CDR' (PDC) library. This novel library system will have properties that will also enable it to greatly facilitate both the downstream affinity-reagent selection and the maturation processes. Methods for testing this library using microfluidics and emulsion screening are discussed.