ULTRASOUND IMAGING CORE

摘要 核心D：无创成像核心：该核心的目的是提供最先进的体内无创成像方法，用于：（a）了解与母胎环境变化相关的心血管生理适应;（B）提供不依赖于动物处死的定量终点，因此可用于在时间上表征母胎界面和胎儿心血管发育和功能的变化。该核心利用了OHSU在使用非侵入性心血管成像来了解发育中胎儿的血管和心室生理学方面的优势。核心人员将由率先开发造影超声灌注成像的研究人员组成，并将这些技术应用于评估健康和疾病中的微血管重塑，并了解流量-功能关系。该项目的成像核心实验室的开发目的不仅是获得组织学或体外方法无法获得的心血管功能和微血管流量的独特信息，而且还创建了一种具有成本效益的方法，用于评估大型动物模型中胎儿环境的影响，而无需采用安乐死。成像核心的利用已被纳入每个主要项目，从而可以预测核心服务的分配。然而，OHSU非侵入性心血管成像研究实验室的优势在于能够适应合作用户成像技术的需求，以获得对分子表型和细胞生物学的独特理解。预计将根据个别研究计划的动态需求开发成像或新技术的其他应用。此外，心脏功能评价将是核心提供的服务，因此每个项目都将有一个完整的灌注和功能评价。每个项目都将利用核心。核心将应用该领域的新技术，因为它们可以为3个项目中的应力模型中的胎儿心脏和胎盘评估带来越来越多的力量。

Abstract Core D: Non-invasive Imaging Core: The purpose of this core is to provide state-of-the-art in vivo noninvasive imaging approaches for: (a) understanding the cardiovascular physiologic adaptations related to changes in maternal-fetal environment; and (b) to provide quantitative end-points that do not rely on animal sacrifice and, hence, can be used to temporally characterize changes in the maternal-fetal interface and fetal cardiovascular development and function. This core takes advantage of OHSU's strength in using noninvasive cardiovascular imaging for understanding vascular and ventricular physiology in the developing fetus. The core personnel will be composed of investigators who have pioneered the development of contrast ultrasound perfusion imaging and have applied these techniques to evaluate microvascular remodeling in health and disease and to understand flow-function relations. The imaging core lab for this project has been developed for the purpose of not only gaining unique information on cardiovascular function and microvascular flow that is not available by histology or ex vivo methods, but also to create a cost-efficient method for evaluating the impact of fetal environment in a large animal model without recourse to euthanasia. Utilization of the imaging core has been incorporated into each ofthe major projects, allowing for predictable allocation of core services. However, a strength of the non-invasive cardiovascular imaging research laboratory at OHSU has been the ability to adapt to the needs of collaborating users imaging technology in order to gain unique understanding of molecular phenotype and cell biology. It is anticipated that additional applications of imaging or new technologies will be developed according to the dynamic needs ofthe individual research programs. In addition, evaluation of cardiac function will be a service provided by the core so that each project will have an entire perfusion and functional evaluation. Each project will take advantage of the core. The core will apply the ever new technologies in the field as they become available to bring increasingly more power to the evaluation ofthe fetal heart and placenta in the stress models in the 3 projects.