New Console and Cold Probe for the Duke 600 MHz NMR Spectrometer System

描述(由申请人提供)：杜克大学提议用新的控制台、低温系统和1H{13C/15N}5 mm 13C增强型冷探头来更换、更新和增强其600 MHz核磁共振波谱系统的故障部件。600 MHz光谱仪安装在杜克大学核磁共振波谱中心内并由其管理，该中心为所有大学研究项目以及研究三角地区和东南部地区的大学和政府实验室的科学家提供核磁共振资源。它支持、促进和培训科学家将核磁共振用于基础生化和生物医学科学的前沿研究。需要所需的新设备部件来取代已有12年历史的控制台和已有8年历史的低温系统和探测器，这些系统和探测器现在以不可接受的速度发生故障。这些部件故障严重影响了许多研究人员的研究活动，他们依赖杜克600 MHz仪器来进行他们资助的项目。该工具的申请超过了24/7的时间表，订阅量很大。然而，最近，由于控制台和我们的第一代冷冻系统和冷探头的关键部件随着老化而出现故障，可用时间有限。瓦里安Inova控制台不再生产，已经过时两代，并被新一代安捷伦DirectDrive2光谱仪取代，这加剧了可用于研究的仪器时间的限制。我们系统的新部件不再制造和翻新，旧部件通常也不能立即获得。我们的600 MHz核磁共振系统所需的新设备组件提供了一种经济高效的策略来恢复和增强这台主力光谱仪，其成本大约是新系统的一半，因为我们的14.09T牛津磁体状态良好，性能优异，一致性好，漂移可以忽略不计。通过对Duke 600 MHz核磁共振光谱仪的升级，目前以下领域的研究活动将得到加强：蛋白质组装研究；蛋白质构象动力学和折叠；参与合成的膜相关脂类和酶；酶结构表征和重新设计；蛋白质的细胞内核磁共振谱；与艾滋病毒疫苗设计相关的免疫原肽和磷脂的抗体识别；细胞信号；以及快速获取多维核磁共振数据的方法开发。

DESCRIPTION (provided by applicant): Duke University proposes to replace, update, and enhance failing components of its 600 MHz NMR spectroscopy system with a new console, cryo-system, and 1H{13C/15N} 5mm 13C-enhanced cold probe. The 600 MHz spectrometer is housed in and is managed by the Duke NMR Spectroscopy Center that serves as an NMR resource for all University research projects and for scientists at universities and government laboratories in the Research Triangle and the Southeastern region. It supports, facilitates and trains scientists in the use of NMR for frontier research in basic biochemical and biomedical sciences. The requested new equipment components are needed to replace a 12-year-old console and 8-year-old cryo- system and probe that are now failing at an unacceptable rate. These component failures have significantly impacted research activities of numerous investigators who depend on the Duke 600 MHz instrument to pursue their funded projects. The instrument is fully subscribed with requests that exceed a 24/7 schedule. Recently, however, the available time has been limited due to unanticipated maintenance and repairs associated with failure of critical components in the console and our first generation cryo-system and cold probe as they have aged. This limitation in the instrument time available for research is compounded by the fact that the Varian Inova console is no longer manufactured, is two generations out of date, and has been replaced by a new generation Agilent DirectDrive2 spectrometer. New parts for our system are no longer manufactured and refurbished, used parts, are often not immediately available either. The new equipment components requested for our 600 MHz NMR system provides a cost effective strategy to restore and enhance this workhorse spectrometer at a cost of approximately half that of a new system since our 14.09T Oxford magnet is in excellent condition and performs with superior homogeneity and negligible drift. Current research activities in the following areas will be enhanced by this upgrade to the Duke 600 MHz NMR spectrometer: studies of protein assemblies; protein conformational dynamics and folding; membrane associated lipids and enzymes involved in their synthesis; enzyme structural characterization and redesign; in-cell NMR spectroscopy of proteins; antibody recognition of immunogenic peptides and phospholipids associated with HIV vaccine design; cellular signaling; and methods development for rapid acquisition of multidimensional NMR data.