Phenomics Core (Page 243)

项目总结(见说明)： 为了支持链中心及其研究人员的目标和目标，需要先进的基于质谱学的分析。在Chain中心提出的目标涵盖了广泛的关键主题，包括生物标记物的发现，巨噬细胞在神经元损伤中的作用的评估，以及中枢神经系统穿透性抗病毒药物的必要性。这些研究计划是为了在艾滋病毒感染及其与老龄化的关系的慢性背景下，促进我们对神经艾滋病的了解、预防和治疗。在这里，我们将提供最先进的蛋白质组学和代谢组学技术，以实现对与以下各项相关的广泛蛋白质和代谢物的识别、量化和关联 神经艾滋病。应对这一挑战对于理解现在改变的疾病进展以及诊断和治疗工具的开发是不可或缺的。我们的方法和分析将允许识别与神经艾滋病及其模型相关的特定蛋白质和代谢物。其中一个期望是，蛋白质组学实验的结果将与代谢组学数据相结合，通过使用这一核心中的分析工具，行政核心的生物信息学小组和系统生物学核心的合作努力将产生综合的数据收集、共享和分析能力，这些能力是 同时潜水和集成，不仅使我们的研究人员能够满足他们的科学目标，而且为神经艾滋病创造了一个独特的资源。这将在整个核心和中心内完成，并直接适用于在检查艾滋病毒对中枢神经系统的影响方面进行的体外、体内和体外临床翻译研究。这些具体目标旨在满足中心的行动计划，两位核心董事不仅带来了蛋白质组学和代谢组学方面的重要专业知识，而且对艾滋病毒及其神经生物学有很好的了解，并拥有神经艾滋病研究的经验，这有助于链中心调查人员的成功。分析仪器和科学能力是这一核心的蛋白质组学和代谢组学组成部分之间的补充。两位主任都将促进和促进Chain Center调查人员与岩心之间的沟通，以确保使用最优化的实验方法和仪器。

PROJECT SUMMARY (See instructions): In order to support the goals and of the CHAIN Center and its investigators advanced mass spectrometric based analyses are needed. The aims proposed in the CHAIN Center cover a broad range of critical topics including biomarker discovery, assessment of the role of macrophages in neuronal damage, and the need for CNS penetrating antiviral drugs. These invesfigations are planned in order to advance our knowledge, prevention and treatment of neuroAIDS in the now chronic setting of HIV infection, and its relationship to aging. Here we will provide state of the art proteomic and metabolomics technologies to enable the identification, quantification, and correlation of the broad range of proteins and metabolites relevant to neuroAIDS. Meeting this challenge is integral to understanding the now altered progression of disease and the development of diagnostic and treatment tools. Our methods and analyses will permit the identification of specific proteins and metabolites associated with neuroAIDS and its models. One of expectations is that results of proteomics experiments will be integrated with metabolomics data, and through the use of analysis tools in this Core, the Bioinformatics subunit of the Administrative Core and the Systems Biology Core a collaborative effort will result in integrated data collection, sharing and analysis capabilities that are simultaneously divers and integrated, not only enabling our investigators to meet their scientific aims but generating a unique resource for neuroAIDS. This will be accomplished within the Core and Center as a whole and is directly applicable to in vitro, in vivo and ex vivo clinical translational studies performed in the examination of the effects of HIV on the CNS. The Specific Aims are designed to meet the Center's Action Plan, and the two Core Directors not only bring significant expertise in proteomics and metabolomics but also have a good understanding of HIV and its neurobiology, and experience in neuroAIDS studies, which facilitate the likelihood of success of CHAIN Center investigators. Analytical instrumentation and scientific capabilities are complementary among proteomics and metabolomics components of this Core. Both directors will promote and facilitate communication between the CHAIN Center investigators and the cores to insure that the most optimal experimental approaches and instrumentation will be used.