Southeast Resource Center for Integrated Metabolomics (SECIM)

产品说明：东南综合代谢组学资源中心（SECIM）整合了现有的优势，为基础和临床科学家创建了一个全面的资源，以获得最先进的代谢组学数据和分析。SECIM与合作伙伴Sanford-Burnham（奥兰多，弗拉）提供基于MS和NMR的代谢组学服务，他也为推广和推广做出了贡献。我们将提供一个完全集成的统计分析平台， 由我们的生物信息学中心生物标志物将通过NMR和MS进行鉴定，其中包括与佛罗里达州立大学的Alchweiler实验室进行从头结构预测的新方法，以及与帝国理工学院尼科尔森小组进行的NMR/MS联合分析。格鲁吉亚大学的Prestegard实验室将为SECIM用户提供动态核极化（DNP）底物服务，我们的合作伙伴AMRIS设施将使用该底物监测活体动物的代谢。SECIM用户还将能够通过我们与NextGen Metabolomics和Thermo Fisher的合作，使用LC/MS进行同位素比值离群值分析（IROA）实验，以测量响应外部扰动或突变的全球代谢组学变化。SECIM将使用MALD 1成像（MS）和HRMAS（NMR）技术提供离体组织分析，用户还可以通过UF临床和转化科学研究所生物储存库访问广泛的组织和血库。SECIM技术核心：1）全球和目标代谢组学的质谱服务（加勒特和Gardell，Co-PI）2）用于全局代谢组学和生物标志物鉴定的NMR光谱学（Edison，PI）3）用于生物标志物鉴定、成像质谱和IROA的高级MS（Yost，PI）4）用于SECIM管道开发和分析的生物信息学（McIntyre，PI）推广和推广核心（Conlon和Smith，Co-PI）通过扩大用户群和提供SECIM能力的教育和培训，将所有内容整合在一起。行政核心协调所有活动，并将制定和实施一个可持续的商业模式，将在第6年及以后自给自足。

DESCRIPTION: The Southeast Resource Center for Integrated Metabolomics (SECIM) integrates existing strengths to create a comprehensive resource for basic and clinical scientists to obtain state-of-the-art metabolomics data and analyses. SECIM offers services in MS- and NMR-based metabolomics with partner Sanford-Burnham (Orlando, Fla.), who also contributes to Promotion & Outreach. We will offer a fully integrated platform for statistical analysis created by our Bioinformatics Core. Biomarkers will be identified by NMR and MS, with new methods for de novo structure prediction with the Bruschweiler lab at Florida State University and joint NMR/MS analysis with the Nicholson group at Imperial College. A service will be provided to SECIM users by the Prestegard lab at the University of Georgia for dynamic nuclear polarization (DNP) substrates that will be used by our partner, the AMRIS Facility, for monitoring of metabolism in living animals. SECIM users will also be able to conduct isotopic ratio outlier analysis (IROA) experiments to measure global metabolomic changes in response to external perturbations or mutations using LC/MS through our partnership with NextGen Metabolomics and Thermo Fisher. SECIM will provide ex vivo tissue analysis using MALDl-imaging (MS) and HRMAS (NMR) techniques, and users can also access an extensive tissue and blood bank through the UF Clinical and Translational Science Institute Biorepository. SECIM technical cores: 1) Mass Spectrometry Services for global and targeted metabolomics (Garrett and Gardell, Co-PIs) 2) NMR spectroscopy for global metabolomics and biomarker identification (Edison, PI) 3) Advanced MS for biomarker identification, imaging mass spectrometry, and IROA (Yost, PI) 4) Bioinformatics for SECIM pipeline development and analysis (Mclntyre, PI) The Promotion & Outreach Core (Conlon and Smith, Co-PIs) brings everything together by expanding the user base and providing education and training in SECIM capabilities. The Administrative Core coordinates all activities and will develop and implement a sustainable business model that will be self-supporting in years 6 and beyond.