Southeast Resource Center for Integrated Metabolomics (SECIM)

描述：东南综合代谢组学资源中心(SECIM)整合了现有的优势，为基础和临床科学家创建了一个全面的资源，以获取最先进的代谢组学数据和分析。SECIM与合作伙伴Sanford-Burnham(佛罗里达州奥兰多)合作提供基于MS和核磁共振的代谢组学服务，Sanford-Burnham也参与了推广和外联活动。我们将为创建的统计分析提供一个完全集成的平台 由我们的生物信息学核心。生物标志物将通过核磁共振和质谱学进行鉴定，使用新方法与佛罗里达州立大学的布鲁奇韦勒实验室进行从头结构预测，并与帝国理工学院的尼科尔森小组进行联合核磁共振/质谱分析。佐治亚大学的Prestegard实验室将向SECIM用户提供动态核极化(DNP)底物服务，我们的合作伙伴AMRIS设备将使用该底物来监测活动物的新陈代谢。SECIM用户还将能够通过我们与NextGen代谢组学和Thermo Fisher的合作，使用LC/MS进行同位素比率离群分析(IROA)实验，以测量全球代谢组变化，以响应外部扰动或突变。SECIM将使用MALDl成像(MS)和HRMAS(核磁共振)技术提供体外组织分析，用户还可以通过UF临床和翻译科学研究所生物库访问广泛的组织和血库。SECIM技术核心：1)全球和靶向代谢组学的质谱学服务(Garrett和Gardell，Co-PI)2)全球代谢组和生物标记物鉴定的核磁共振波谱(Edison，PI)3)生物标记物鉴定、成像质谱学和IROA(Yost，PI)4)SECIM管道开发和分析的生物信息学(Mclntyre，PI)推广和外联核心(Conlon and Smith，Co-Pis)通过扩大用户基础并提供SECIM能力方面的教育和培训，将一切整合在一起。行政核心协调所有活动，并将制定和实施一个可持续的业务模式，该模式将在第六年及以后自给自足。

DESCRIPTION: The Southeast Resource Center for Integrated Metabolomics (SECIM) integrates existing strengths to create a comprehensive resource for basic and clinical scientists to obtain state-of-the-art metabolomics data and analyses. SECIM offers services in MS- and NMR-based metabolomics with partner Sanford-Burnham (Orlando, Fla.), who also contributes to Promotion & Outreach. We will offer a fully integrated platform for statistical analysis created by our Bioinformatics Core. Biomarkers will be identified by NMR and MS, with new methods for de novo structure prediction with the Bruschweiler lab at Florida State University and joint NMR/MS analysis with the Nicholson group at Imperial College. A service will be provided to SECIM users by the Prestegard lab at the University of Georgia for dynamic nuclear polarization (DNP) substrates that will be used by our partner, the AMRIS Facility, for monitoring of metabolism in living animals. SECIM users will also be able to conduct isotopic ratio outlier analysis (IROA) experiments to measure global metabolomic changes in response to external perturbations or mutations using LC/MS through our partnership with NextGen Metabolomics and Thermo Fisher. SECIM will provide ex vivo tissue analysis using MALDl-imaging (MS) and HRMAS (NMR) techniques, and users can also access an extensive tissue and blood bank through the UF Clinical and Translational Science Institute Biorepository. SECIM technical cores: 1) Mass Spectrometry Services for global and targeted metabolomics (Garrett and Gardell, Co-PIs) 2) NMR spectroscopy for global metabolomics and biomarker identification (Edison, PI) 3) Advanced MS for biomarker identification, imaging mass spectrometry, and IROA (Yost, PI) 4) Bioinformatics for SECIM pipeline development and analysis (Mclntyre, PI) The Promotion & Outreach Core (Conlon and Smith, Co-PIs) brings everything together by expanding the user base and providing education and training in SECIM capabilities. The Administrative Core coordinates all activities and will develop and implement a sustainable business model that will be self-supporting in years 6 and beyond.