A 800 MHz Nuclear Magnetic Resonance Spectrometer in Support of Life Science Rese

描述（由申请人提供）：本申请要求为纽约北部地区的生命科学研究专用的800 MHz核磁共振（NMR）光谱仪提供资金。拟议的工具将支持9个NIH资助的主要用户（共持有11个NIH研究赠款以支持所述项目）以及7个重要的次要用户（共持有7个NIH和其他联邦机构的赠款）的研究计划。用户组包括来自纽约州立大学北部医科大学、锡拉丘兹大学和纽约州立大学ESF的研究人员，所有这些研究人员都位于纽约州锡拉丘兹，以及来自纽约州伊萨卡市康奈尔大学、纽约州罗切斯特市罗切斯特大学和纽约州布法罗市豪普特曼伍德沃德研究所的研究人员。拟议仪器支持的研究领域包括但不限于蛋白质和RNA结构、折叠和相互作用。在用户实验室研究的生物分子涉及与人类健康和疾病相关的各种过程，例如能量转导，RNA结构和mRNA剪接，基于蛋白质和RNA的生物传感器的开发，肿瘤发生，神经元功能和神经变性，内分泌和外分泌信号传导，组蛋白基因转录的调节和膜运输。目前，在参与机构附近没有800 MHz仪器，因此需要极高场NMR应用的研究人员必须将样品带到或发送到国家设施之一，如位于威斯康星州麦迪逊的NMRFAM或位于华盛顿州里奇兰的太平洋西北国家实验室。相对较长的周转时间以及旅行成本和运输敏感样品的困难限制了这些非现场替代品的效率，从而产生了对具有esy访问的本地设施的更大需求。申请资金的仪器在灵敏度和光谱分辨率方面都是最先进的，因此可以分析蛋白质的结构、动力学和相互作用 和超出目前可用仪器可能的大小范围的核酸。还要求提供资金，以购买一个5毫米低温冷却探头，以获得最高灵敏度，从而能够分析丰度低或溶解度差的样品。所要求的仪器配备有四个通道，用于进行三重共振实验，同时进行氘的去耦，用于分析（部分）氘化样品。该仪器将安置在纽约州立大学环境科学与林业学院（SUNY ESF）现有的NMR设施中，由设施经理大卫·基姆勒先生管理和维护。Kiemle先生在过去的25年里一直管理着SUNY ESF NMR设施，在生物大分子的NMR分析以及现代NMR光谱仪的维护和维修方面拥有丰富的经验。收购800 MHz NMR光谱仪将为纽约北部地区提供最先进的仪器，这将加速NIH资助的结构生物学和药物设计领域的生物医学研究，如果我们想找到在分子水平上对抗人类疾病的方法，这些研究领域是必不可少的。

DESCRIPTION (provided by applicant): This application requests funding for an 800 MHz nuclear magnetic resonance (NMR) spectrometer dedicated for life science research in the Upstate New York area. The proposed instrument will support the research programs of 9 NIH funded major users (holding a total of 11 NIH research grants in support of the described projects) as well as 7 significant minor users (holding a total of 7 grants from the NIH and other federal agencies). The group of users includes researchers from SUNY Upstate Medical University, Syracuse University and SUNY ESF, all located in Syracuse, NY as well as researchers from Cornell University, Ithaca, NY, University of Rochester, Rochester, NY and the Hauptman Woodward Institute, Buffalo, NY. Research areas supported by the proposed instrument include, but are not limited to, protein and RNA structure, folding and interactions. The biomolecules studied in the user's laboratories are involved in a variety of processes relevant to human health and disease such as energy transduction, RNA structure and mRNA splicing, development of protein and RNA based biosensors, oncogenesis, neuronal function and neurodegeneration, endocrine and exocrine signaling, regulation of histone gene transcription, and membrane transport. There is currently no 800 MHz instrument near the participating institutions so that researchers with a need for very high field NMR applications have to bring or send samples to one of the national facilities such as NMRFAM in Madison, WI or the Pacific Northwest National Labs in Richland, WA. The relatively long turnaround times as well as the cost for traveling and the difficulties with shipping sensitive samples limit the efficiency of these offsite alternatives, creating an ever-greater need for a local facility with esy access. The instrument for which funding is requested is forefront both regarding sensitivity and spectral resolution, thus allowing the analysis of structure, dynamics and interactions of proteins and nucleic acids beyond a size range of what is currently possible with the available instrumentation. Funding is also requested for a 5 mm cryogenically cooled probe for ultimate sensitivity allowing the analysis of samples with low abundance or poor solubility. The requested instrument is equipped with four channels for conducting triple resonance experiments with simultaneous decoupling of deuterium for analyzing (partially) deuterated samples. The instrument will be housed in the existing NMR facility at SUNY College of Environmental Science and Forestry (SUNY ESF), where it will be managed and maintained by the facility manager, Mr. David Kiemle. Mr. Kiemle has managed the SUNY ESF NMR facility for the past 25 years and has extensive experience with NMR analysis of bio macromolecules as well as maintenance and repair of modern NMR spectrometers. Acquisition of an 800 MHz NMR spectrometer will provide the Upstate New York region with a state-of-the-art instrument that will accelerate NIH funded biomedical research in the areas of structural biology and drug design, research areas that are essential if we want to find ways to fight human disease on a molecular level.