Leica Laser Microdissection Microscope for a Shared Resource

描述（由申请人提供）：激光捕获显微切割已成为从组织或细胞培养物中分离特定细胞群的主要研究技术，可在高度纯化的细胞而不是异质组织上进行进一步的亚细胞技术。该提案要求支持购买Leica LMD 7000激光捕获显微镜，并将其保存在机构共享资源“分子病理学核心”中。 获得这种仪器对两个主要用户组的工作至关重要，即佛罗里达大学免疫学和移植中心以及炎症生物学和外科科学实验室。 管理、维护和高水平技术援助和培训将由分子病理学中心经验丰富的工作人员提供。所有用户培训和生物安全问题都已得到解决。强有力的机构承诺是由医学院院长，博士迈克尔好，和高级副院长，博士史蒂芬Sugrue，和病理学，免疫学和实验室医学系主席，博士迈克尔克莱尔Salzler的支持证明。Leica LMD 7000被选为最适合多用户机构的仪器，因为它具有更大的灵活性，适用于各种样品类型，并且明显满足了未满足的需求，因为该机构没有通过共享资源提供这种仪器。Leica仪器具有几个理想的功能，包括直观简单的软件，可自动进行样品切割，4管提取功能，明场和荧光，细胞培养，以及用于锐利激光切割的上级物镜。本提案中强调的主要用户由NIH提供充足的资金，并获得了需要该仪器的积极和待定的赠款。每个机构都将从机构获得激光捕获仪器中受益匪浅。玛莎·坎贝尔·汤普森（Martha Campbell-Thompson）和马克·阿特金森（Mark Atkinson）将使用LMD 7000研究浸润淋巴细胞和胰岛，以了解1型糖尿病的β细胞破坏和胰岛再生机制。克莱顿马修斯将进行研究的基本生物学线粒体在β细胞和改变葡萄糖代谢通过基因组分析分离胰岛。Laurence Morel研究了系统性狼疮模型中的各种免疫细胞群，目前正在将激光捕获研究分包给另一家机构的合作者。Lyle Moldawer研究了脓毒症和全身炎症反应综合征中宿主对急性炎症过程的反应，以及对慢性炎症过程的反应，包括癌症和艾滋病相关的恶病质。他也有国家卫生研究院的积极资助，用于动脉静脉瘘的激光捕获显微切割研究。最后，该工具将通过这一共享资源提供给其他调查人员，并采用收费服务机制进行培训和辅助或无辅助使用。

DESCRIPTION (provided by applicant): Laser capture microdissection has become a primary research technique to isolate specific cell populations from tissues or cell culture affording further sub-cellular techniques to be conducted on highly purified cells rather than a heterogeneous tissue. This proposal requests support for the purchase of a Leica LMD7000 laser capture microscope, to be maintained in an institutional Shared Resource, the Molecular Pathology Core. Access to this instrumentation is crucial to the work of two major user groups, namely, Center for Immunology and Transplantation and Laboratory of Inflammation Biology and Surgical Sciences, at the University of Florida. Administration, maintenance, and high-level technical assistance and training will be provided by experienced staff in the Molecular Pathology Core. All user training and biosafety issues have been addressed. Strong institutional commitment is demonstrated by the support of College of Medicine Dean, Dr. Michael Good, and Senior Associate Dean, Dr. Steven Sugrue, and Department of Pathology, Immunology and Laboratory Medicine Chairman, Dr. Michael Clare-Salzler. The Leica LMD7000 was selected as the most suitable for a multi-user facility due to its greater flexibility for a wide range of sampe types and clearly fulfills an unmet need as the institution does not have this instrumentation available through a shared resource. The Leica instrument has several ideal features including intuitive and simple software to automate sample dissection, 4-tube extraction capabilities, brightfield and fluorescence, cell culture, and superior objectives for sharp laser cutting. The major users highlighted in this proposal are well-funded by the NIH with active and pending grants requiring this instrumentation. Each would benefit greatly from having institutional access to a laser capture instrument. Martha Campbell-Thompson and Mark Atkinson will use the LMD7000 to study infiltrating lymphocytes and islets to understand mechanisms of beta cell destruction and islet regeneration for type 1 diabetes. Clayton Mathews will conduct research on the basic biology of mitochondria in beta cells and altered glucose metabolism through genomic analyses of isolated islets. Laurence Morel studies various immune cell populations in a model of systemic lupus and is currently subcontracting the laser capture studies to a collaborator at another institution. Lyle Moldawer studies host responses to acute inflammatory processes in sepsis and systemic inflammatory response syndromes and to chronic inflammatory processes, including cancer and AIDS-associated cachexia. He too has active NIH funding for studies with laser capture microdissection for arterial venous fistula. Finally, the instrument will be availabl to other investigators through this shared resource using a fee-for-service mechanism for training and assisted or unassisted use.