Mechanims of Breast Development and Carcinogenesis

病理核心(核心B)将支持方案内的多个项目，为以下项目提供人体组织 搜索。病理核心主任安德里亚·理查森博士是一名董事会认证的病理学家，拥有 乳房病理学专业。她是布里格姆妇女医院乳房组织库的主管 并监督乳房组织的收集、储存、处理和分析。乳房组织储存库 BWH拥有超过1500个带有完整病理注释的乳腺肿瘤标本。这些标本中有许多 进行了广泛的分子分析，包括基因表达阵列分析、SNP阵列 基因分型，也出现在石蜡包埋的组织微阵列中。除了肿瘤组织， BWH资料库还收集乳房缩小手术中的正常乳房组织样本。这些 正常组织以冰冻样本的形式储存，也可以作为新鲜样本提供，用于分离正常组织 器官或乳房干细胞。 病理核心将为干细胞研究(Polyak)和3D器官提供正常的乳腺组织 培养(布鲁日)。将提供新鲜的肿瘤样本以建立小鼠的原发肿瘤移植 温伯格)。此外，理查森博士将参与病理比较，包括分子和 组织学，在亲代人类肿瘤和由此产生的异种移植之间。基因表达阵列数据 从人类肿瘤中获得的将用于BRCA1相关通路的系统相互作用分析 (利文斯顿)。理查森博士将提供组织和科学专业知识来分析冷冻肿瘤 表达BRCA1基因产物的标本和石蜡组织微阵列，包括IRIS (利文斯顿)。RELEVANCE(参见说明)： 使用真实的人类乳腺组织和乳腺肿瘤材料进行的研究有望提供最大的 关于女性乳腺癌发病机制和肿瘤行为的相关解答。 项目/

The Pathology Core (Core B) will support multiple projects within the program by providing human tissues for esearch. The director of the Pathology Core, Dr. Andrea Richardson, is a board certified pathologist with a specialty in breast pathology. She is director of the breast tissue bank at Brigham and Women's Hospital and oversees the collection, storage, processing and analysis of breast tissues. The breast tissue repository at BWH holds over 1500 breast tumor specimens with full pathology annotation. Many of these specimens have undergone extensive molecular analysts including gene expression array profiling, SNP array genotyping, and are also represented in paraffin-embedded tissue microarrays. In addition to tumor tissues, he BWH repository also collects normal breast tissue specimens from breast reduction surgeries. These normal tissues are stored as frozen samples or can be provided as fresh samples for isolation of normal organoids or breast stem cells. The pathology core will provide normal breast tissue for stem cell studies (Polyak) and for 3-D organoid cultures (Brugge). Fresh tumor samples will be provided to establish primary tumor xenografts in mice Weinberg). In addition, Dr. Richardson will participate in the pathologic comparisons, both molecular and histology, between the parent human tumors and the resultant xenografts. Gene expression array data obtained from human tumors will be used in system interaction analyses of BRCA1 related pathways (Livingston). Dr. Richardson will provide both tissue and scientific expertise to analyze frozen tumor specimens and paraffin tissue microarrays for the expression of BRCA1 gene products, including IRIS (Livingston).RELEVANCE (See instructions): Research using actual human breast tissue and breast tumor material will hopefully provide the most relevant answers to pathogenesis and tumor behavior of breast cancer in women. PROJECT/