Protein Expression Core

东北生物防御中心的许多项目都需要高质量的纯化重组蛋白 蛋白质和假型病毒进行研究。蛋白质表达核心的目标是促进 本申请的各个主题中提出的调查、开发和试点研究。工作人员 核心将协助研究者将他们的研究转化为质量控制的标准测定， 疫苗或治疗剂。工作人员将与研究人员合作开发蛋白质的表达系统 它们的研究需要使用商业载体或定制的表达载体。该设施将 提供完整的蛋白表达和纯化服务。这将包括获得完善的 沃兹沃斯中心的细菌表达能力;利用哺乳动物， 昆虫表达和体外翻译在阿尔伯特爱因斯坦医学院;和生产 耶鲁大学专门设计的假型VSV病毒，极大地促进了中和抗体的分析 大学这三个服务部门由各自领域的专家领导， 为研究项目提供试剂，并为NBC提供灵活性，以支持广泛的 对主要调查员不断变化的要求迅速作出反应， 改变NIAID的任务。此外，核心将保持蛋白质和表达的稳定库存 应变，以便在紧急情况下，这些材料的生产将是迅速的。

Many projects within the Northeast Biodefense Center application require high-quality purified recombinant proteins and pseudotyped virus for their research. The goal of the Protein Expression Core is to facilitate the investigative, developmental, and pilot studies proposed in the various themes of this application. The staff of the Core will assist investigators in the translation of their studies to quality controlled standard assays, vaccines or therapeutics. Staff will work with investigators to develop expression systems for the proteins required for their studies either using commercial vectors or customized expression vectors. The facility will provide a complete protein expression and purification service. This will include access to well established bacterial expression capabilities at the Wadsworth Center; specialized capabilities exploiting mammalian, insect expression, and in vitro translation at the Albert Einstein College of Medicine; and the production of specially designed pseudotyped VSV viruses that greatly facilitate analysis of neutralizing antibodies at Yale University. These three service components, headed by experts within their field, will allow the rapid provision of reagents for research projects and provide the NBC with the flexibility to support the wide range of existing needs and to respond rapidly to the changing requirements of principal investigators and changing NIAID mandates. In addition, the Core will maintain stable stocks of proteins and expression strains so that in a time of emergency, production of these materials will be rapid.