Integrating biological and engineering approaches to maximize the utility of baculoviruses as gene delivery vehicles

整合生物和工程方法，最大限度地发挥杆状病毒作为基因传递载体的效用

• 批准号: 355513-2012
• 负责人: Aucoin, Marc
• 金额: $ 1.46万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=595680
• 关键词: Integrating; biological; engineering; approaches; maximize

The demand for biologics is increasing at an unprecedented rate. US demand alone for biologics is expected to go beyond $100 billion/year by 2015. Animal cell culture is the major platform for high value biologics production, with Chinese Hamster Ovary cells having dominated the bioprocess industry for many years. Insect cells, however, offer many advantages over mammalian cells. These advantages include: not propagating human or mammalian specific viruses; being grown in suspension culture to high cell densities without the presence of serum in the media - eliminating a potential source of prion and mammalian virus contamination; and, in combination with the baculovirus expression vector system (BEVS), allowing high levels of protein expression.

对生物制剂的需求正以前所未有的速度增长。预计到2015年，仅美国对生物制剂的需求就将超过1000亿美元/年。动物细胞培养是高价值生物制品生产的主要平台，中国人卵巢细胞多年来一直主导着生物加工行业。然而，昆虫细胞提供了许多优于哺乳动物细胞的优势。这些优势包括：不繁殖人类或哺乳动物特异性病毒;在培养基中不存在血清的情况下在悬浮培养物中生长至高细胞密度-消除朊病毒和哺乳动物病毒污染的潜在来源;以及与杆状病毒表达载体系统（BEVS）组合，允许高水平的蛋白质表达。