Fluorescence microscopy Shared Resource

荧光显微镜（FM）共享资源提供广泛的显微镜服务， 亨斯迈癌症研究所（HCI）成员和整个犹他州大学研究界。的 共享资源的主要重点是提供最先进的仪器、软件和专业知识 活细胞和固定细胞成像所必需的。该设施有两个奥林巴斯FV1000光谱共焦 显微镜、两个FV300共焦镜、一个FVX 2Photon共焦镜、一个Pathway 855高通量共焦镜、一个 用于低光活细胞成像的Yokagowa旋转盘共聚焦，以及两台配备 数码相机为图像的3D渲染、量化和反卷积提供计算支持（例如， Improvision的Velocity，Bitplane的Imaris）。调频资源提供培训， 通过直接援助和通过正式的大学课程使用当前的技术和设备。 FM共享资源为HCI实验室科学计划（核控制）的成员提供服务 细胞生长和分化;细胞反应和调节;成像，诊断和治疗; 和胃肠道癌症），使他们能够可视化细胞形态和行为以及分布 在正常和疾病状态下，分子进入细胞区室。FM共享资源 增强了对基因表达、细胞凋亡和细胞分裂的研究。它允许表征肿瘤生长 和形态学。 Chris Rodesch博士在一名工作人员的协助下指导资源。仪表和 FM共享资源的工作人员被安置在一个中心位置。 FM共享资源是一个由大学和HCI监督的机构管理的设施 领导有一个教师咨询委员会和资源审查用户满意度， 年度调查。该设施以按服务收费的方式运营（按存储容量计费）。使用FM共享 癌症中心成员的资源与同行评审的资金是54%。请拨资金来自 CCSG支付拟议资源预算的15%（44，733美元）。FM共享资源具有 为癌症中心成员提供额外使用的充足容量。

The Fluorescence Microscopy (FM) Shared Resource provides a broad range of microscopy services for Huntsman Cancer Institute (HCI) members and the entire University of Utah research community. The primary focus of the Shared Resource is to provide state-of-the-art instrumentation, software, and expertise necessary for the imaging of live and fixed cells. The facility has two Olympus FV1000 spectral confocal microscopes, two FV300 confocals, an FVX 2Photon confocal, a Pathway 855 high-throughput confocal, a Yokagowa spinning disk confocal for low-light live cell imaging, and two widefield microscopes equipped with digital cameras. Computational support for 3D rendering, quantification, and deconvolution of images (e.g.. Velocity from Improvision, Imaris from Bitplane) is also provided. The FM Resource provides training in the use of current techniques and equipment by direct assistance and through formal University courses. The FM Shared Resource serves members of the HCI laboratory-based scientific Programs (Nuclear Control of Cell Growth and Differentiation; Cell Response and Regulation; Imaging, Diagnostics, and Therapeutics; and Gastrointestinal Cancers), enabling them to visualize cell morphology and behavior as well as distribution of molecules into cellular compartments in both normal and diseased states. The FM Shared Resource enhances studies of gene expression, apoptosis, and cell division. It allows characterization of tumor growth and morphology. Chris Rodesch, PhD, directs the Resource with the assistance of one staff member. The instrumentation and staff of the FM Shared Resource are housed in a central location. The FM Shared Resource is an institutionally managed facility with supervision from both University and HCI leadership. There is a Faculty Advisory Committee and the Resource is reviewed for user satisfaction by annual surveys. The facility is operated on a fee-for-service basis (chargeback). Use of the FM Shared Resource by Cancer Center members with peer-reviewed funding is 54 percent. Funds are requested from the CCSG to cover 15 percent ($44,733) of the proposed Resource budget. The FM Shared Resource has ample capacity for additional use by Cancer Center members.