X-Aptamer Selection Kit

描述：AM生物技术公司(AM)正在开发一种易于使用的试剂盒，使生命科学实验室的技术人员能够以合理的价格快速开发可再生的X-适体(XA)亲和试剂。亲和试剂广泛应用于研究、诊断和临床应用。抗体是目前最常见的亲和试剂；然而，生命科学市场的方方面面都遇到了问题，包括聚集、沉淀、难以质量控制、批次到批次的重复性问题、保质期短、需要冷藏、生产成本高和永久性变性。X-适配子解决了每一种抗体限制。X-适配子选择试剂盒有三个基本组件：1)基于微珠的寡核苷酸文库；2)一组试剂，用于向感兴趣的靶选择XA；以及)用于XA选择的几个简单硬件项目。X-适配子由DNA支架组成，它结合了非DNA功能基团，如氨基酸侧链和小分子，它们与靶标的相互作用比标准DNA更强烈。XA是在一种专有的、单轮的、基于珠子的选择过程中开发的，使用的设备几乎可以在任何生命科学实验室找到。该方法能够选择不能酶法生成的化学修饰的亲和剂，这为一系列以前从未用于基于适体的亲和剂选择的化学官能团扫清了道路。到目前为止，已经测试了几个化学官能团，并导致了具有低皮莫结合亲和力的X-适配子。此外，使用XA工艺，小分子的结合亲和力提高了一百万倍。尽管到目前为止已经取得了非凡的结果，但迫切需要评估和优化官能团的组合，这些官能团有可能创造出具有无与伦比的亲和力、专一性、物理稳定性和强大的可制造性的蛋白质亲和试剂。在该项目中，AM将开发和 评估用于选择不同蛋白质靶标的XA的多种化学修饰的文库设计，并将开发用于选择配体修饰的XA与配体结合的蛋白质靶标的可靠方法。这一第一阶段项目的成功完成将为第二阶段项目奠定基础 优化X-适配子选择试剂盒流程，以便在第三阶段实现商业化。

DESCRIPTION: AM Biotechnologies (AM) is developing an easy-to-use kit that enables a technician in a life science laboratory to rapidly develop renewable X-Aptamer (XA) affinity reagents at a reasonable price. Affinity reagents are widely used in research, diagnostic, and clinical applications. Antibodies are currently the most common affinity reagents; however, every aspect of the life science market has experienced problems with them including aggregation, precipitation, difficult quality control, batch-to-batch reproducibility issues, short shelf life, eed for cold storage, costly production, and permanent denaturation. X-Aptamers address every antibody limitation. The X-Aptamer Selection Kit has three basic components: 1) a microbead-based oligonucleotide library; 2) a set of reagents for selecting XAs to a target of interest; and ) a few simple hardware items used for XA selection. X-Aptamers are comprised of a DNA scaffold that incorporates non-DNA functional groups such as amino acid side chains and small molecules that interact with a target more robustly than standard DNA. XAs are developed in a proprietary, single-round, bead-based selection process using equipment found in virtually any life science laboratory. The process enables the selection of chemically-modified affinity reagents that cannot be enzymatically generated, which clears the way for a wide array of chemical functional groups that have never before been used in aptamer-based affinity agent selections. Several chemical functional groups have been tested to date and have resulted in X-Aptamers with low picomolar binding affinity. In addition, using XA processes, the binding affinity of a small molecule was improved by a million-fold. While extraordinary results have been obtain to date, there is a strong need to evaluate and optimize combinations of functional groups that hold the potential for creating protein affinity reagents with unmatched affinity, specificity, physical stability, and robust manufacturability. In this project, AM will develop and evaluate multiple chemically-modified library designs for selecting XAs to diverse protein targets and will develop robust methods for selecting ligand-modified XAs to ligand-binding protein targets. Successful completion of this Phase I project will set the stage for a Phase II project to optimize the X-Aptamer Selection Kit processes for commercialization in Phase III.