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X-Aptamer Selection Kit

X 适体选择试剂盒

基本信息

批准号：
8894157
负责人：
Curtis Honming Lam
金额：
$ 20万
依托单位：
AM BIOTECHNOLOGIES, LLC
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-09-01 至 2015-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8894157
关键词：
Address Affinity Amino Acids Antibodies Aromatic Amino Acids Aspartate Binding Binding Proteins Biological Sciences Biotechnology Cell Nucleus Characteristics Charge Chemicals Chemistry Communities Cryopreservation DNA Diagnostic Diagnostics Research Ensure Equipment Exercise Frequencies Generic Drugs Glutamates Guanidines Indoles Investigation Isoelectric Point Laboratories Lead Libraries Life Ligand Binding Ligands Marketing Medical Methods Microspheres Modification Nucleic Acids Nucleotides Oligonucleotides Performance Pharmaceutical Preparations Phase Phenols Post-Translational Protein Processing Precipitation Price Process Production Protein Binding Proteins Protocols documentation Quality Control Reagent Reproducibility Retinol Binding Proteins Route Side Specificity Staging System Testing Texas Therapeutic Tryptophan Tyrosine Universities Vertebral column aptamer arginyllysine base chemical group clinical application commercialization design experience folate-binding protein functional group improved inter-alpha-inhibitor interest phase 2 study phosphorodithioic acid public health relevance research and development scaffold small molecule time use

项目摘要

DESCRIPTION: AM Biotechnologies (AM) is developing an easy-to-use kit that enables a technician in a life science laboratory to rapidly develop renewable X-Aptamer (XA) affinity reagents at a reasonable price. Affinity reagents are widely used in research, diagnostic, and clinical applications. Antibodies are currently the most common affinity reagents; however, every aspect of the life science market has experienced problems with them including aggregation, precipitation, difficult quality control, batch-to-batch reproducibility issues, short shelf life, eed for cold storage, costly production, and permanent denaturation. X-Aptamers address every antibody limitation. The X-Aptamer Selection Kit has three basic components: 1) a microbead-based oligonucleotide library; 2) a set of reagents for selecting XAs to a target of interest; and ) a few simple hardware items used for XA selection. X-Aptamers are comprised of a DNA scaffold that incorporates non-DNA functional groups such as amino acid side chains and small molecules that interact with a target more robustly than standard DNA. XAs are developed in a proprietary, single-round, bead-based selection process using equipment found in virtually any life science laboratory. The process enables the selection of chemically-modified affinity reagents that cannot be enzymatically generated, which clears the way for a wide array of chemical functional groups that have never before been used in aptamer-based affinity agent selections. Several chemical functional groups have been tested to date and have resulted in X-Aptamers with low picomolar binding affinity. In addition, using XA processes, the binding affinity of a small molecule was improved by a million-fold. While extraordinary results have been obtain to date, there is a strong need to evaluate and optimize combinations of functional groups that hold the potential for creating protein affinity reagents with unmatched affinity, specificity, physical stability, and robust manufacturability. In this project, AM will develop and evaluate multiple chemically-modified library designs for selecting XAs to diverse protein targets and will develop robust methods for selecting ligand-modified XAs to ligand-binding protein targets. Successful completion of this Phase I project will set the stage for a Phase II project to optimize the X-Aptamer Selection Kit processes for commercialization in Phase III.

产品说明：AM Biotechnologies（AM）正在开发一种易于使用的试剂盒，使生命科学实验室的技术人员能够以合理的价格快速开发可再生的X-Aptamer（XA）亲和试剂。亲和试剂广泛用于研究、诊断和临床应用。抗体是目前最常见的亲和试剂;然而，生命科学市场的每个方面都经历了它们的问题，包括聚集、沉淀、难以质量控制、批次间重现性问题、短保质期、需要冷藏、生产成本高和永久变性。X-适体解决了每种抗体限制。X-Aptamer选择试剂盒有三个基本组件：1）基于微珠的寡核苷酸文库; 2）一组用于将XA选择到目标靶标的试剂;以及）用于XA选择的一些简单硬件项目。 X-Aptamer由DNA支架组成，其中包含非DNA功能基团，例如氨基酸侧链和小分子，与靶点的相互作用比标准DNA更强烈。XA是在一个专有的，单轮，珠为基础的选择过程中使用的设备，发现在几乎任何生命科学实验室。该方法使得能够选择不能酶促产生的化学修饰的亲和试剂，这为以前从未用于基于适体的亲和试剂选择的广泛的化学官能团扫清了道路。迄今为止，已经测试了几种化学官能团，并产生了具有低皮摩尔结合亲和力的X-适体。此外，使用XA过程，小分子的结合亲和力提高了一百万倍。虽然迄今为止已经获得了非凡的结果，但仍然强烈需要评估和优化官能团的组合，这些官能团具有产生具有无与伦比的亲和力、特异性、物理稳定性和稳健的可制造性的蛋白质亲和试剂的潜力。在这个项目中，AM将开发和评估多种化学修饰的文库设计，用于选择针对不同蛋白质靶标的XA，并将开发用于选择针对配体结合蛋白质靶标的配体修饰的XA的稳健方法。第一阶段项目的成功完成将为第二阶段项目奠定基础，优化X-Aptamer选择试剂盒的工艺，以便在第三阶段实现商业化。