B7-H1 Expressing Macrophages Mediate Immunosupression in Glioma

描述(申请人提供)：局部免疫抵抗和全身性免疫抑制是胶质瘤有效免疫治疗的主要障碍。免疫应答在很大程度上依赖于肿瘤特异性CD8+细胞溶解T细胞，并可通过诱导T细胞凋亡而被抑制。B7-同源1(B7-H1)是胶质瘤细胞表面的一种蛋白，与T细胞上的程序性死亡1(PD-1)受体结合，可诱导无能或凋亡。肿瘤相关巨噬细胞被认为通过抗原递呈和释放特定的细胞因子来促进局部免疫反应。最近的证据表明，巨噬细胞可以两极分化为促炎(M1)或抗炎(M2)表型，这是由其级联的细胞因子定义的。此外，这一证据表明，肿瘤相关巨噬细胞可以在细胞表面表达B7-H1，并可以诱导活化的T细胞凋亡，而不依赖于胶质瘤细胞。巨噬细胞介导的细胞溶解T细胞反应的抑制可能是胶质瘤患者局部和全身免疫耐受的主要因素。我们将提供初步证据：1)胶质母细胞瘤患者外周血和肿瘤中巨噬细胞表面B7-H1的表达增加，2)胶质瘤细胞可以通过可溶性因子刺激外周血单核细胞表达B7-H1，3)表达B7-H1的单核细胞诱导CTL凋亡，4)胶质瘤细胞刺激单核细胞产生IL-10，5)IL-10足以激活单核细胞中B7-H1的表达。基于这些数据，我们假设来自胶质瘤的肿瘤来源的可溶性因子诱导肿瘤相关巨噬细胞产生IL-10，而IL-10通过自分泌信号激活B7-H1的表达。在这项建议中，我们将评估B7-H1在肿瘤相关巨噬细胞上的时空分布与肿瘤负荷的关系，并探讨B7-H1表达的诱导机制。具体地说，我们将研究IL-10自分泌信号的作用和STAT3途径的激活，以评估它们在这一过程中的参与。我们还将采用一种无偏见的蛋白质纯化方法来鉴定导致单核细胞上B7-H1上调的胶质瘤衍生因子。

DESCRIPTION (provided by applicant): Local immunoresistance and systemic immunosuppression represent major impediments to effective immunotherapy for gliomas. The immune response to vaccination is largely dependent on tumor specific CD8+ cytolytic T cells, and can be suppressed by induction of T cell apoptosis. B7-Homologue 1 (B7-H1) is a surface protein on glioma cells that binds to the programmed death 1 (PD-1) receptor on T cells and can induce anergy or apoptosis. Tumor-associated macrophages are thought to contribute to the local immune response through antigen presentation and the release of specific cytokines. Recent evidence suggests that macrophages can be polarized to pro-inflammatory (M1) or anti-inflammatory (M2) phenotypes, defined by their cascade of cytokines. In addition, this evidence demonstrates that tumor-associated macrophages can express B7-H1 at the cell surface and can induce apoptosis of activated T cells, independent of glioma cells. Macrophage- mediated suppression of the cytolytic T cell response may be a primary factor in the local and systemic immunoresistance seen in glioma patients. We will provide preliminary evidence that 1) B7-H1 expression on macrophages is increased in peripheral blood and tumor from glioblastoma patients, 2) glioma cells can stimulate B7-H1 expression in peripheral monocytes through a soluble factor, 3) B7-H1 expressing monocytes induce CTL apoptosis, 4) glioma cells stimulate monocytes to produce IL-10, and 5) IL-10 is sufficient to activate B7-H1 expression in monocytes. Based on these data, we hypothesize that tumor-derived soluble factors from gliomas induce IL-10 production in tumor-associated macrophages, which activates B7- H1 expression through autocrine signaling. In this proposal we will evaluate the spatiotemporal distribution of B7-H1 on tumor-associated macrophages relative to tumor burden and investigate the mechanisms by which B7-H1 expression is induced. Specifically, we will study the role of IL-10 autocrine signaling and activation of the STAT3 pathway to evaluate their involvement in this process. We will also take an unbiased protein purification approach to identify the glioma-derived factor responsible for upregulation of B7-H1 on monocytes.