Label-free fluorescence lifetime imaging for intraoperative real-time guidance of neurological procedures

无标记荧光寿命成像，用于神经系统手术的术中实时指导

• 批准号: 10529315
• 负责人: Orin Bloch
• 金额: $ 54.47万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-12-15 至 2025-11-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10529315
• 关键词: Affect; Area; Biochemical; Biometry; Biopsy; Brain; Brain Neoplasms; Cauterize; Characteristics; Classification; Clinical; Clinical Research; Clinical Trials; Complement; Complex; Comprehensive Cancer Center; Conventional Surgery; Craniotomy; Data; Data Display; Databases; Diagnostic; Diagnostic Imaging; Diagnostic Procedure; Discrimination; Engineering; Environment; Epilepsy; Equipment; Excision; Fluorescence; Glioma; Goals; Harvest; Hemostatic function; Histopathology; Human; Image; Image-Guided Surgery; Imaging Device; Imaging Techniques; Imaging technology; Intervention; Label; Letters; Magnetic Resonance Imaging; Malignant Neoplasms; Malignant neoplasm of brain; Manufacturer; Measurement; Metabolic; Microscope; Necrosis; Neoplasm Metastasis; Neurodegenerative Disorders; Neurologic; Neuronavigation; Neurosurgeon; Neurosurgical Procedures; Normal tissue morphology; Operative Surgical Procedures; Optics; Outcome; Pathologic; Pathology; Patient-Focused Outcomes; Patients; Predictive Value; Procedures; Property; Protocols documentation; Qi; Radiation necrosis; Resources; Scanning; Signal Transduction; Specimen; Surgeon; Surgical Oncology; Surgically-Created Resection Cavity; System; Techniques; Technology; Testing; Time; Tissue Sample; Tissues; Tumor Tissue; Validation; Visual; Visualization; brain abnormalities; brain tissue; brain tumor resection; cohort; data acquisition; diagnostic value; feature detection; flexibility; fluorescence lifetime imaging; image processing; imaging system; improved; instrumentation; learning progression; multidisciplinary; nervous system disorder; neuro-oncology; neuropathology; neurosurgery; novel; optical imaging; pragmatic implementation; prospective; real-time images; standard of care; success; temporal measurement; tool; tumor

PROJECT SUMMARY/ABSTRACT The outcome of brain tumor surgery is critically dependent on the neurosurgeon's ability to distinguish between abnormal and normal tissue in real-time. Our goal is to enhance this discrimination by using label-free Fluorescence Lifetime Imaging (FLIm) to detect tissue biochemical and metabolic characteristics that distinguish among different tissue types and, by integrating FLIm into the neurosurgical workflow, to provide this information in a real-time, visual format useful for guiding tumor biopsy and resection. FLIm-derived tissue fluorescence feature information will be co-registered with the preoperative-MRI (pMRI) and projected onto the conventional surgical microscope field-of-view (FOV). This should improve delineation of tumor margins and thus increase both the diagnostic yield of brain biopsy and the extent of tumor resection. The proposed FLIm technique will incorporate the following features: (1) Safe, rapid, and simultaneous measurement of time-resolved fluorescence decays in multiple spectral emission bands that will acquire extensive information in one scanning measurement of a large area of tissue selected by the surgeon; and (2) Fast analysis, display, and augmentation of fluorescence parameters that enable real-time visualization of optical data encoding diagnostic information onto the surgical FOV. The proposed clinical studies using FLIm as a stand- alone tool will establish classifiers to correlate FLIm parameters with specific tissue pathologies, an important step in demonstrating FLIm's diagnostic value. The proposed integration of FLIm as an adjunct to the neuronavigation system and surgical microscope will provide data for combined analysis to validate the benefit of FLIm diagnostics in neurosurgical procedures. Three aims are proposed: Aim 1) Construct and integrate a FLIm device as a diagnostic adjunct with conventional neurosurgical tools. Aim 2) Clinically evaluate the relationship between FLIm parameters and distinct tissue pathologies and develop classifiers for different tissue types. Aim 3) Validate FLIm for real-time intraoperative guidance through a prospective analysis. In summary, this study will demonstrate the clinical feasibility and utility of FLIm for intraoperative real-time assessment of neurosurgical margins and the resulting improvement in neuropathologic diagnostic yield. The acquired FLIm parameter database will enable subsequent clinical trials for automated tissue classification and diagnostic prediction. The new FLIm instrumentation, characterized by simple, fast and flexible data acquisition and display, and its seamless integration with existing neurosurgical imaging, will provide a less expensive alternative to intraoperative MRI and a valuable complement to current standard-of-care diagnostic procedures. Success in this area will warrant a more generalized use of FLIm in surgical oncology (other cancers) as well as guided interventions for treatment of functional neurologic diseases (e.g. epilepsy, neurodegenerative diseases).

项目总结/文摘