Phase 2 REAL-TIME MONITORING OF TRACE AMOUNTS OF PESTICIDES IN WATER AND FOOD USI

描述(由申请人提供)：开发第2阶段测试版，用于在树顶果汁加工厂演示现场适用的实时远程监测传感器设备，能够同时检测未过滤果汁、液体或废水中的多种农药。我们建议进一步开发碳纳米管基质活性生物传感器领域，以有效固定多种农药，以及传感器的保存和制造规范。我们的第一阶段工作导致了一个自动化实时系统的原型，并在检测阿特拉津、毒死蜱和马拉硫磷时展示了从1ppt到10ppm的灵敏度和非常低的交叉反应。 首席研究员：安妮·施瓦茨，首席执行官，分包PI‘s：杰夫·詹金斯博士协助：克雷格·马库斯博士、古特尔·施奈德博士和伊桑·米诺特·奥苏博士。 第二阶段(传感器)：这项工作将利用基于排列的多壁碳纳米管的超灵敏电化学传感平台进行农药的伏安分析。我们将检测基于碳纳米阵列的传感单元的能力和性能，并开发同时连续检测多种农药。这项工作的一部分将是保存和保质期测试 传感器和优化，用于废水和食品加工厂的自动化使用。我们将使用计算物理学来预测纳米材料结合的粘合强度，以进行系统优化。 第二阶段(系统)：进一步开发电子硬件，以制造80个传感器的多路复用一次性阵列，使用针对电子微流控泵机构的专有设计，自动向测试传感器输送和清除流体样本，以及为食品加工生产线和废水系统的过程控制发出警报的无线数据传输。 该项目在产品开发和商业化中的应用：问题的陈述我国的食品和水质量处于危险之中，因为我们负担不起使用现有方法采样和分析水所需的人力和时间。杀虫剂带来的风险被严重低估了。NOAA渔业局和华盛顿州立大学最近对2009年美国地质调查进行的一项研究表明，多种农药的联合影响对健康的危害比通过分析每种农药单独影响的结果估计的要大得多(1)。事实上，当幼年鲑鱼同时只接触两种杀虫剂时，它们会立即死亡。这些都是被认为是安全的常见杀虫剂。这些杀虫剂与儿童的ADHD有关(2)。缺乏为公众健康提供全面的杀虫剂测试的能力是不受限制的 到俄勒冈州；这是一个全国性的问题。 解决方案：现场适用的实时远程监测设备检测水和食品中的农药，将使食品加工商、DEQ、EPA和NIEH研究人员能够获得足够的数据，了解环境和工业或农业因素之间的关系，以及影响水和食品质量的具体机制，从而实现变化。目前没有足够的数据来制定全面和有效的农药管理计划。此外，食品加工者还需要对ppt进行实时、灵敏的测量，以及时调整工艺，以降低食品中的农药含量。Dahl Natural、亚利桑那州立大学和俄亥俄州立大学正在合作展示这项技术的可行性，以便将其商业化，为这一威胁健康和环境的问题提供解决方案。 商业应用和其他好处：该解决方案的独特之处在于，该设备能够在水上环境中部署一到两年，而不需要维修，直到该时间段结束。此外，数据可以同时远程发送到太多不同的数据库。因此，NIEH的商业应用和对公众的好处很多，包括食品加工、废水监测和警报系统、超级基金现场监测、及时发放个人废水排放许可证、为NIEH、DEQ、EPA和大学研究人员建立一个全面的数据库。 农药检测市场每年超过10亿美元。传感器的低成本和显著的单位成本节约 测试(从一个平均样本的每个杀虫剂每天200美元到600美元，到每天50多个平均样本，最低只需30美元)，并增加对互联网/内联网可访问数据的访问。 关键词：水中农药远程监测，电化学传感器，自组装多壁碳纳米管，抗体偶联，马拉硫磷，毒死蜱，阿特拉津。 国会议员总结：NIEH资助了一项开创性的研究工作，这项工作可以极大地提高我们国家探测食物、饮用水、湖泊、河流和海洋水环境威胁的能力。这项新技术将能够一次远程监测有毒农药1-2年。

DESCRIPTION (provided by applicant): Development of a phase 2 beta for demonstration in Tree Top juice processing plant of a Field Applicable Real-time Remote Monitoring Sensor Device capable of detecting multiple pesticides simultaneously in unfiltered juice, fluid or waste water. We propose to further develop the carbon nanotube matrices active biosensor area for effective immobilization of multiple pesticides, as well as preservation and manufacturing specifications of the sensors. Our phase 1 work resulted in a prototype for an automated real time system and has demonstrated sensitivities from 1ppt to 10ppm with very low cross reactivity in the detection of Atrazine, Chloropyrifos, and Malathion. Principal Investigator: Anne Schwartz CEO DN, Subcontract PI's: Dr. Jeff Jenkins OSU. Assisting: Dr. Craig Marcus, Dr. Guenter Schneider and Dr. Ethan Minot OSU. Phase 2 (The Sensor): This work, will utilize an ultra sensitive electrochemical sensing platform based on aligned MWCNT for voltammetric analysis of pesticides. We will examine the capability and performance of the carbon nano-array based sensing unit and develop simultaneous and continuous detection of multiple pesticides. Part of this work will be preservation and shelf life testing of the sensor and optimization for automated use in waste water and food processing plants. We will use computational physics to predict adhesion strength of nanomaterial binding for system optimization. Phase 2 (The System): Further development of electronic hardware to fabricate a 80 sensor multiplexed disposable array, automated delivery and clearance of fluid samples to the test sensor using a proprietary design for an electronic microfluidic pump mechanism and wireless data transmission for alerts to process controls of food processing lines and waste water systems. Application of the Project to Product Development and Commercialization: Statement of the problem; our nation's food and water quality is at risk since we cannot afford the man power and time it takes to sample and analyze water using current methods. The risk pesticides pose has been grossly underestimated. A recent study, of the U.S. 2009 Geological survey done by NOAA Fisheries Service and Washington State University, indicates that the combined effect of multiple pesticides is far more dangerous to the health than was estimated by analyzing the results for the effect of each pesticide individually(1). In fact when juvenile salmon were exposed to only two pesticides at once they immediately died. These were common pesticides that were thought to be safe. These pesticides are linked to ADHD in children (2).This lack in capability of providing comprehensive pesticide testing for the health of the public is not limited to Oregon; it is a national problem. Solution: A field applicable real-time remote monitoring device to detect pesticides in water and food would allow Food Processors, DEQ, EPA, and NIEH researchers to get enough data on the relationship between environmental and industrial or agricultural factors and specific mechanisms that effect water and food quality, to effect change. Currently there is not enough data to develop comprehensive and effective plans for managing pesticides. Also real-time, sensitive to ppt, measurement is needed for food processors to adjust processes in time to lower the amount of pesticides in food. Dahl Natural, ASU and OSU are partnering to demonstrate feasibility of this technology so that it can be commercialized to provide a solution to this health and environmental threatening problem. Commercial Applications and Other Benefits: Unique to this solution is the ability of this device to be deployed in an aquatic environment for from one to two years without the need for servicing until the end of that time period. In addition the data can be remotely sent too many different data bases at once. Hence the commercial application and benefits to the NIEH and the public are many and include food processing, waste water monitoring and alarm systems, superfund site monitoring, timely individual wastewater discharge permits issued, establishment of a comprehensive data base for NIEH, DEQ, EPA and University researchers. The market for pesticide testing is over 1 Billion dollars a year. The low cost of the sensors and dramatic cost saving per test (from $200 to $600 a day per pesticide for one mean sample to over 50 mean samples per day for as little as $30.00) as well as increase access to internet /intranet accessible data. Key Words: Remote Monitoring of Pesticides in Water, Electrochemical Sensor, Self-assembled MWCNT, and antibody based conjugation, Malathion, Chloropyrifos, Atrazine. Summary for Members of Congress: The NIEH has funded a ground breaking research effort which could dramatically improve our nation's ability to detect threats to food, drinking water, lakes, rivers and ocean aquatic environments. This new technology will be able to remotely monitor toxic pesticides for 1-2 years at a time.