GENERAL SCIENTIFIC

一般科学核心B的目的是协助研究者进行动物手术、电生理学、 免疫细胞化学、透射电子显微镜和图像分析要求 项目教员。核心还协助研究人员进行各种生化分析和病毒载体 生产核心设施包括执行拟定研究所需的人员和设备 在项目1到3中。该中心将进行一般动物准备，包括麻醉、插管， 导管插入术、温度监测和生理变量的调节。核心会协助 研究人员在准备动物的创伤性脑损伤，如个别项目中所述。在 此外，还可以利用专门知识来修改未来可能需要的实验动物制剂， 调查事务所核心人员将进行灌注、固定和移除大脑，以获得光和电子 显微镜检查核心人员将进行免疫细胞化学程序，以及开发 新的程序可视化的额外的抗体。核心将组织加工成塑料 包埋用于光学和电子显微镜分析。高质量的薄切片将由 该核心内的人员，并染色用于超微结构分析。建立图像分析程序 使用无偏体视学程序来量化免疫反应性细胞的频率，以及 确定将使用的挫伤区域和体积。

The purpose of the General Scientific Core B is to assist investigators in animal surgery, electrophysiology, immunocytochemistry, transmission electron microscopy, and image analysis requirements for the Program Project faculty. The Core also assists investigators in various biochemical analyses and viral vector production. The Core facilities consist of personnel and equipment needed to perform the proposed studies in Projects 1 through 3. This Core will conduct general animal preparation, including anesthesia, intubation, catheterization, temperature monitoring, and regulation of physiological variables. The Core will assist investigators in preparing the animals for traumatic brain injury, as described in the individual projects. In addition, expertise is available to modify experimental animal preparations that may be required for future investigations. Core personnel will perform perfusion, fixation, and removal of brains for light and electron microscopic examination. Core personnel will carry out immunocytochemical procedures, as well as develop new procedures for the visualization of additional antibodies. The Core will process tissue for plastic embedding for both light and electron microscopic analysis. High quality thin sections will be produced by personnel within this Core and stained for ultrastructural analysis. Established image analysis procedures using non-biased stereological procedures to quantify the frequency of immunoreactive cells, as well as to determine contusion areas and volumes will be utilized.