Regulation of FGF signaling in lacrimal gland development

项目摘要 本项目的长期目标是研究泪腺中FGF信号的调节 发展，这对了解患病泪腺的病因学有重要意义， 人类泪腺通过主要由FGF驱动的分支形态发生过程发育 信号传导，其本身由细胞外空间中的糖胺聚糖调节。该应用程序将重点 糖胺聚糖在泪腺发育中的作用，并确定糖胺聚糖 精细结构调节FGF信号传导。具体来说，将开发小鼠遗传模型来测试 糖胺聚糖生物合成基因在泪腺发育中的功能，生化检测将 检查糖胺聚糖和FGF分子之间的相互作用，最后， 培养系统将用于确定FGF信号传导中糖胺聚糖的需求。通过 研究小鼠泪腺中FGF信号的调节，该项目将有助于 治疗人类先天性眼病的医学研究，并促进我们对 间质-上皮相互作用范例。

PROJECT SUMMARY The long term objective of this project is to investigate the regulation of FGF signaling in lacrimal gland development, which has important implications for understanding the etiology of the diseased lacrimal gland in human. The lacrimal gland develops through a branching morphogenesis process primarily driven by FGF signaling, which itself is regulated by glycosaminoglycan in the extracellular space. This application will focus on the role of glycosaminoglycan in lacrimal gland development and determine how the glycosaminoglycan fine structure regulates FGF signaling. Specifically, mouse genetic models will be developed to test the functions of glycosaminoglycan biosynthetic genes in lacrimal gland development, biochemical assays will be performed to examine the interaction between glycosaminoglycan and FGF molecules, and finally, explant culture systems will be utilized to determine the requirement of glycosaminoglycan in FGF signaling. By investigating the regulation of FGF signaling in murine lacrimal gland, this project will both contribute to medical research in treating human congenital eye diseases and advance our understanding of the mesenchymal-epithelial interaction paradigm.