Genetically Engineered Mouse Models for TMEN Research

核心B将提供一个恒定的资源，小鼠窝藏黑色素瘤，表达 BRAF*，包括对BRAFi敏感或耐药的肿瘤活检对。综合实力 该核心在于与核心A中来自人黑素瘤的平行样品相互作用和交叉， 提供遗传元件的跨物种三角测量， 表型效应该核心将不仅提供Braf* 小鼠（iBIP和M *）黑素瘤组织库， 以及在基线、治疗后和复发时收集的衍生细胞系， 离体设计新的等位基因并进行临床前治疗研究。至免疫活性的 IBIP黑色素瘤的背景和天然肿瘤微环境设置对此带来了关键特征 TMEN中心。

Core B will provide a constant resource of mice harboring melanomas that express BRAF*, including pairs of tumor biopsies that are sensitive or resistant to BRAFi. The integrative power of this core lies in interacting and intersecting with parallel samples from human melanomas in Core A, providing cross-species triangulation of genetic elements that confer tumor microenvironment-specific phenotypic effects. This core will provide not only a bank of Braf* mouse (iBIP and M^) melanoma tissues and derivative cell lines collected at baseline, post-treatment and upon relapse, but also capability to engineer new alleles ex vivo and perform preclinical therapeutic studies. The immunocompetent background and natural tumor microenvironment setting of IBIP melanomas bring a key feature to this TMEN center.