FY 2013 Contract HHSN271201300012C, SBIR-1, Topic 003, Automated Instrument for C

在SBIR I期资助申请中，Codex BioSolutions提出构建一种基于细胞的高通量检测方法，使用新型染料m-MPI测量化学毒性。这种染料的设计克服了许多 目前用于测量线粒体膜电位的染料的缺点。母体染料JC-1先前已用于此目的。在线粒体中，它作为聚集体聚集，发出红色荧光，而在细胞质中，它作为单体，发出绿色荧光。然而，JC-1难以使用，因为它具有非常低的水溶性，这使得难以使用其差异荧光特性。我们的染料m-MPI具有增加的溶解度，允许简单地通过计算红色/绿色荧光的比率来容易地定量评估MMP状态。m-MPI也可以与不与JC-1一起工作的细胞（例如原代细胞）一起使用，这至少部分是由于其增加的灵敏度。我们相信我们的检测方法将在更短的时间内以更低的成本更准确地评估危害。

In this SBIR phase I grant application, Codex BioSolutions proposes to construct a cell-based high throughput assay that measures chemical toxicity using a novel dye, m-MPI. This dye was engineered to overcome many of the drawbacks of current dyes used to measure mitochondrial membrane potential. The parent dye JC-1 has been used for this purpose previously. In the mitochondria, where it accumulates as aggregates, it fluoresces red, whereas in the cytoplasm where it is a monomer, it fluoresces green. However, JC-1 is difficult to use because it has very low water solubility which makes using its differential fluorescent properties difficult. Our dye, m-MPI has increased solubility allowing easy quantitative assessment of MMP status simply by calculating the ratio of red/green fluorescence. m-MPI can also be used with cells that do not work with JC-1 (such as primary cells) due at least in part to its increased sensitivity. We believe our assay will more accurately assess hazards in a shorter time and with less cost.