MEMS Based Intraoperative Photoacoustic Imaging

描述（由申请人提供）：保乳手术，或乳房肿瘤切除术，是早期诊断为浸润性乳腺癌的患者最常见的外科手术。明确肿瘤边缘和完全手术切除肿瘤是预防局部复发和提高无病生存率的关键。目前，没有准确的方法来确定肿瘤边缘术前或术中。由于肿瘤边缘呈阳性，大约20%到30%接受乳房肿瘤切除术的患者需要额外的手术切除任何剩余的肿瘤。为了开发一种检测乳腺组织肿瘤边缘的灵敏方法，我们提出设计一种肿瘤靶向纳米颗粒成像探针，并利用基于微机电系统（MEMS）的光声断层扫描（PAT）实现术中成像。本研究的目的是：1)开发一种基于MEMS的双频光声断层成像系统，用于术中肿瘤成像；2)通过模拟实验和体内实验对纳米探针- mems - pat系统进行验证和优化；3)评价纳米探针- mems - pat在体内引导下切除小鼠乳腺肿瘤的效果。基于微型化MEMS的敏感、高分辨率术中光声成像系统的开发，对于术中确定肿瘤边缘，预防肿瘤复发，从而提高乳腺癌患者的生存率具有很大的潜力。

DESCRIPTION (provided by applicant): Breast-conserving surgery, or lumpectomy, is the most common surgical procedure for patients diagnosed with an early stage of invasive breast cancer. Clearly defined tumor margins and complete surgical removal of tumors are critical for preventing local recurrence and increasing disease-free survival. Currently, there is no accurate method to identify tumor margins pre- or intra-operatively. About 20% to 30% of the patients who undergo lumpectomy require additional surgery to excise any remaining tumors due to the presence of positive tumor margins. To develop a sensitive approach for detecting tumor margins in breast tissues, we propose to engineer a tumor targeted nanoparticle imaging probe and to realize the intraoperative imaging using microelectromechanical systems (MEMS) based photoacoustic tomography (PAT). The objectives of this proposed study are: 1) To develop a MEMS based dual-frequency photoacoustic tomography system for intraoperative tumor imaging; 2) To validate and optimize the nanoprobe-MEMS-PAT system using phantom and in vivo experiments; and 3) To evaluate the efficacy of in vivo nanoprobe-MEMS-PAT guided resection of mouse mammary tumors. The development of a sensitive and high resolution miniaturized MEMS based intraoperative photoacoustic imaging system should have great potential for determining tumor margins during surgery, preventing tumor recurrence and therefore, increasing the survival rate of breast cancer patients.