The Role of Sulfiredoxin in Tumorigenesis and Cancer Progression.

硫氧还蛋白(SRX)是一个新的基因，已被发现在几种类型的人类中高表达 癌症，包括皮肤癌和肺癌。然而，SRX在肿瘤发生发展中的作用尚未被 研究得很好。这项研究的主要假设是SRX具有致癌功能，可能有助于 小鼠和人的肿瘤发生和癌症进展。这项研究的长期目标是 了解SRX在人类肿瘤发生发展中的作用和分子机制， 这将对未来癌症预防和治疗的新策略的开发具有价值。第一 这项研究的目的是测试SRX基因的缺失是否会导致小鼠对肿瘤产生更强的抵抗力 采用化学诱导小鼠皮肤癌变模型。第二个目的是研究它的功能和 SRX在人肺癌细胞迁移、侵袭和转移中的分子基础。第三个目标是 鉴定与SRX相互作用的蛋白质，并确定它们对致癌功能的贡献 SRX基因在人肺癌细胞中的表达。SRX的功能将通过细胞培养和小鼠模型进行检测。 皮肤癌变，以及人类癌细胞发生和转移的小鼠模型。机械论 研究将集中在细胞信号变化和蛋白质-蛋白质相互作用的生物化学和 分子生物学技术。拟议研究的成功完成将进一步推动 了解SRX在人类癌症中的作用，将拓宽对抗氧化蛋白的了解 肿瘤发生和癌症进展，并将促进癌症新策略的开发 预防和治疗。

Sulfiredoxin (Srx) is a novel gene that has been found to be highly expressed in several types of human cancers including cancers of skin and lung. However, the function of Srx in cancer development has not been well studied. The primary hypothesis of this study is that Srx has an oncogenic function that may contribute to tumorigenesis and cancer progression in mouse and human. The long-term object of this study is to understand the function and molecular mechanisms of Srx in human cancer development and progression, which will be valuable for future development of novel strategies for cancer prevention and treatment. The first aim of the study is to test whether the genomic loss of Srx will cause mice more resistant to tumorigenesis using a chemical induced mouse skin carcinogenesis model. The second aim is to study the function and molecular basis of Srx in human lung cancer cell migration, invasion and metastasis. The third aim is to characterize the proteins that interact with Srx and to determine how they contribute to the oncogenic function of Srx in human lung cancer cells. The function of Srx will be examined using cell culture and mouse model of skin carcinogenesis, and mouse models of human cancer cell tumorigenesis and metastasis. The mechanistic study will be focused on the cell signaling change and protein-protein interactions using biochemistry and molecular biology techniques. The successful completion of the proposed research will further the understanding of the role of Srx in human cancer, will broaden the knowledge of antioxidant proteins in tumorigenesis and cancer progression, and will facilitate the development of novel strategies for cancer prevention and treatment.