Regeneration, Repair and Remodeling of the Lower Urinary Tract

描述（由申请人提供）：本申请建立IR-BU规划中心是基于明确的假设，即再生医学技术的更广泛临床应用取决于对相关模型系统中器官再生和修复过程的更好理解。为此，我们组建了一个跨学科的团队，该团队具有独特的资格来追求该规划中心的重点目标。规划中心团队的主要人员反映了研究人员的理想联盟，他们在下尿路的研究和临床翻译方面具有广泛和互补的经验，在干细胞病因学（包括肿瘤形成的原因）、贡献和组成以及干细胞和基因治疗在肝再生和造血中的作用方面具有公认的专业知识。一个教育丰富计划，建议增加机构，国家和国际认可我们的努力，此外，提供一个良好的环境和资源，培养下一代的翻译泌尿外科研究人员。该科学项目专门针对估计有3500万患有膀胱疾病的美国人，其中大多数是慢性疾病（即，感染、先天性疾病和炎症），其最终损害细胞和组织完整性，导致膀胱功能降低，以及数百万患者的生活质量降低。尽管我们鼓励 尽管在大鼠功能性膀胱再生方面取得了进展，但仍然明显的是，需要开发额外的动物模型来加快科学进展向临床转化的步伐。为此，我们建议使用多色移植程序，这将是第一次，允许识别哪些细胞或组织负责更坏的修复/再生过程。因此，我们将把新的成像技术纳入我们正在进行的功能性膀胱再生的研究中，为膀胱壁再生和修复的研究提供一种跨学科的方法，如下所示： 具体目标1：评价和量化介导小鼠模型中功能性膀胱再生的细胞增殖的空间和时间特征。 具体目标#2：确定局部干/祖细胞与骨髓源性干/祖细胞的贡献。

DESCRIPTION (provided by applicant): This application to establish an IR-BU Planning Center is based on the explicit assumption that more widespread clinical applications of regenerative medicine technologies are contingent on an improved understanding of the organ regeneration and repair process in relevant model systems. To this end, we have assembled an interdisciplinary team that is uniquely qualified to pursue the focused aims of this Planning Center. The key personnel on the Planning Center team reflect an ideal union of investigators with extensive and complementary experience in research and clinical translation in the lower urinary tract, with recognized expertise in stem cell etiology), contribution and composition of tumor formation, as well as the role of stem cells and gene therapy in liver regeneration and hematopoiesis. An Educational Enrichment Program is proposed to increase institutional, national and international recognition of our efforts, and moreover, to provide an excellent environment and resources for training the next generation of translational urologic researchers. The scientific project specifically targets the estimated 35 million Americans that suffer from bladder disease, most of which are chronic conditions (i.e., infections, congenital disorders, and inflammation) that ultimately compromise cellular and tissue integrity leading to reduced bladder function, as well as a diminished quality of life for millions of patients. Despite our encouraging advancements in functional bladder regeneration in rats, it remains obvious that development of additional animal models is necessary to accelerate the pace of scientific progress toward clinical translation. To this end we propose the use of multicolored transplant procedures that will, for the first time, allow the identification of what cells or tissue is responsible for the badder repair/regeneration processes. Thus, we will incorporate novel imaging technology into our ongoing studies of functional bladder regeneration to provide an interdisciplinary approach to the study of bladder wall regeneration and repair as follows: Specific Aim #1: Evaluate and quantify the spatial and temporal characteristics of the cellular proliferation that mediates functional bladder regeneration in the mouse model. Specific Aim #2: Determine the contribution of local stem/progenitor cells versus bone marrow-derived stem/progenitor cells.