Development of a discovery platform based on microfluidics and fluorescent cell f

项目描述（由申请人提供）：该项目的总体目标是开发基于微流体和功能细胞分析的发现平台，该平台适用于同时筛选数百种蛋白质或小分子化合物，具有成本效益和高通量。该项目的具体目标是：(1)开发基于微流体的平台，用于高通量筛选潜在的蛋白质和小分子治疗药物。(2)利用荧光2-脱氧葡萄糖（2- dog）模拟物建立胰岛素刺激的3T3-L1脂肪细胞葡萄糖摄取测定。(3)在基于微流体的多孔细胞培养生物芯片中集成并执行功能性2-DOG摄取测定。这个项目的成果是一个具有成本效益的基于微流体的平台，适用于高通量筛选蛋白质和小分子治疗化合物。

DESCRIPTION (provided by applicant): The overall goal of the project is to develop discovery platform based on microfluidics and functional cell assays that is suitable for screening hundreds of proteins or small-molecule compounds simultaneously in a cost-effective and high throughput manner. The projects Specific Aims are: (1) To develop a microfluidic-based platform for high-throughput screening of potential protein and small molecule therapeutics. (2) To develop an insulin stimulated glucose uptake assay in differentiated 3T3-L1 adipocytes using fluorescent 2-deoxy glucose (2-DOG) analog. (3) To integrate and perform a functional 2-DOG uptake assay in the microfluidics based multi-well cell culture biochip. The deliverable from this completed project is a cost-effective microfluidics based platform suitable for high throughput screening of proteins and small molecule therapeutic compounds.