Cellular and Molecular Biology

核心B将促进最先进的细胞和分子生物学技术在所有 计划项目中的项目。核心B的职责将涉及两个独立的但 相关职能：1)组织培养股将提供组织培养细胞和必要的专业知识 完成个人提案中的细胞生物学方案和2)分子生物学单位将 提供试剂和专业知识，以完成五个项目中描述的分子生物学实验。 下面描述的是核心B中使用的主要方法的具体细节。这些方法包括但不限于 TO、逆转录酶(RT)聚合酶链式反应(PCR)、RT实时聚合酶链式反应、Northern、Southern和 蛋白质印迹分析、反义寡核苷酸和siRNAs的研制、基因片段的亚克隆 用于核探针开发、核探针合成和靶向基因阵列分析。这些程序将 在拟议研究过程中根据需要向调查人员提供新的方法 将根据需要进行开发。

Core B will facilitate the use of state-of-the-art cellular and molecular biology technologies in all of the projects in the Program Project. The responsibilities of Core B will involve the performance of two separate but related functions: 1) The Tissue Culture unit will provide tissue culture cells and the expertise necessary to complete the cellular biology protocols in the individual proposals and 2) The Molecular Biology unit will provide reagents and expertise to complete the molecular biology experiments described in the five projects. Described below are specific details of the primary methods used in Core B. These include, but are not limited to, reverse transcriptase (RT) polymerase chain reaction (PCR), RT real-time PCR, Northern, Southern and Western blot analysis, development of antisense oligonucleotides and siRNAs, subcloning of gene fragments for riboprobe development, and riboprobe synthesis, and targeted gene array analysis. These procedures will be provided to the investigators during the course of the proposed studies as required and new methodologies will be developed as needed.