Genomics

基因组学核心的作用是支持该中心开展的研究项目，提供- 一流的转录分析能力，适用于广泛的样本类型。该中心将可以进入 一家在过去8年里一直在运营的设施。它受益于持续的 贝勒医疗保健系统的部分投资，并拥有最先进的基因组 分析仪器和IT基础设施。数据将以高通量和经济高效的方式生成 在严格受控的实验室环境中使用llLumina串珠阵列平台，确保最高的数据质量 和再现性。其他转录特征分析技术也可通过该核心获得，例如 纳米串计数器平台和ABI固态高通量测序仪。一支由 生物信息学家已经整合到核心，以开发和维护数据管理 构成其运营支柱的基础设施。生物信息学和生物统计学支持也将 通过我们的数据挖掘核心向个人调查人员提供。北卡罗来纳大学的基因组学核心 在基因组学和免疫学领域的交界处开展了项目，并取得了显著的成果 分析方面的专门知识：a)各种疾病患者的血液；b)从 组织或细胞培养；c)体外暴露于各种先天刺激的血液。尖端分子 还将提供用于测量microRNA丰度或RNA测序的简档分析。 基因组学核心将与行政、临床样本和数据挖掘核心密切合作。支持 将提供给所有项目。更具体地说，体外血型和体外免疫 将在项目1、3、4和5的背景下生成响应性分析。 将从项目1和2的分离细胞群中产生。RNA-seq和microRNA图谱分析将 将在项目1的范围内进行。

The role of the genomics core is to support the research projects carried out by the center by providing stateof- the-art transcription profiling capabilities over a wide range of sample types. The center will have access to a facility that has been in continuous operation for the past 8 years. It has benefited from a continuous investment on the part ofthe Baylor Health Care System and has at its disposal state ofthe art genomic analysis instruments and IT infrastructure. Data will be generated on the high-through and cost-effective lllumina BeadArray platform in a strictly controlled laboratory environment to insure the highest data quality and reproducibility. Other transcriptional profiling technologies are also available through this core, such as the NanoString nCounter platform and an ABI SOLID high throughput sequencer. A team of bioinformaticians has been integrated to the core in order to develop and maintain the data management infrastructure that constitutes the backbone of its operation. Bioinformatics and biostatistics support will also be made available to individual investigators through our data mining core. The genomics core at BUR has carried out projects at the interface between the fields of genomics and immunology, and gained significant expertise in profiling: a) blood of patients with a wide range of diseases; b) small cell numbers isolated from tissues or cell cultures; c) blood exposed in vitro to a wide range of innate stimuli. Cutting-edge molecular profiling assays for measurement of microRNA abundance or RNA-sequencing will also be available. The genomics core will work closely with the administrative, clinical sample and data mining cores. Support will be provided to all the projects. More specifically, ex-vivo blood profiles and in vitro immune responsiveness assays will be generated in the context of project 1, 3, 4 and 5. Transcriptional profiles will be generated from isolated cell populations for projects 1 and 2. RNA-seq and microRNA profiling assays will be carried out in the context of project 1.