Microarray Core

微阵列核心

• 批准号: 7696469
• 负责人: Damien Chaussabel
• 金额: $ 22.97万
• 依托单位: BAYLOR RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2014-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7696469
• 关键词: Adoption; Algorithms; Animals; Antigens; Arts; Automation; B-Lymphocytes; Bioinformatics; Biological Assay; Biometry; Blood; CD8B1 gene; Cell Count; Cell Culture Techniques; Charge; Clinical Data; Core Facility; Custom; Data; Data Analyses; Data Files; Data Quality; Data Set; Data Sources; Data Storage and Retrieval; Databases; Dendritic Cells; Disease; Environment; Genes; Genetic Transcription; Genomics; Healthcare Systems; Housing; Human; Immune; Immune system; Immunology; In Vitro; Individual; Internet; Investments; Laboratories; Management Information Systems; Mining; Online Systems; Operative Surgical Procedures; Pathway Analysis; Patients; Peptides; Phenotype; Preparation; Process; Property; Reproducibility; Research Infrastructure; Research Personnel; Research Project Grants; Role; Sampling; Secure; Solutions; Stimulus; System; Technology; Tissues; Vaccinated; Vaccines; Vertebral column; Work; cost; cost effectiveness; data acquisition; data management; data mining; good laboratory practice; human subject; improved; instrument; instrumentation; nonhuman primate; technology development; tool

The role of the genomics core is to support the research projects carried out by the center by providing state-of-the-art transcription profiling and data mining capabilities over a wide range of sample types. The center will have access to a facility that has been in continuous operation for the past 7 years. It has benefited from a continuous investment on the part of the Baylor Health Care System and has at its disposal state of the art genomic analysis instruments and IT infrastructure. Data will be generated on the highthrough and cost-effective Illumina BeadArray platform in a strictly controlled laboratory environment to insure the highest data quality and reproducibility. A team of bioinformaticians has been integrated to the core in order to develop and maintain the data management infrastructure that constitutes the backbone of its operation. Custom data management and mining solutions are available for the optimal exploitation of large volumes of microarray data. Bioinformatics and biostatistics support will also be made available to individual investigators for the analysis of their microarray results. The microarray core at BUR has carried out projects at the interface between the fields of genomics and immunology, and gained significant expertise in profiling: a) blood of patients with a wide range of diseases; b) small cell numbers isolated from tissues or cell cultures; c) blood exposed in vitro to a wide range of innate stimuli; and d) blood cultivated in the presence of antigenic peptides. Support will be provided for the following projects: Project 1 (Banchereau): profiling genes expressed in in vitro generated CD8+ CTL; Project 2 (Palucka): a) profiling T and B cells isolated from vaccinated animals; b) profiling human mucosal dendritic cells (DCs) isolated from humouse samples; Project 3 (Sekaly): profiling genes expressed in CD8+ CTL isolated from vaccinated patients; Project 4 (Legrand): a) profiling antigen specific immune-reactivity in the blood of non human primates (NHP), before and after vaccine administration; b) profiling human mucosal DCs isolated from NHP and human samples; Technology development 1 (Zurawski): microarray analyses will be performed in order to study the activation properties of the various DC targeting vaccines on DCs. Technology development 2 (Pascual): antigens specific B cells will be isolated from mucosal samples and analyzed with microarrays to establish their phenotype.

基因组学核心的作用是支持该中心开展的研究项目， 最先进的转录分析和数据挖掘能力，涵盖广泛的样本类型。的 该中心将有权使用一个在过去7年中一直在连续运行的设施。它有 受益于贝勒医疗保健系统的持续投资， 最先进的基因组分析仪器和IT基础设施。数据将在highthrough上生成 在严格控制的实验室环境中， 确保最高的数据质量和再现性。一个生物信息学家小组已经被整合到 核心，以开发和维护构成 其操作。定制的数据管理和挖掘解决方案可用于最佳利用 大量的微阵列数据。生物信息学和生物统计学的支持也将提供给 个体研究者用于分析他们的微阵列结果。BUR的微阵列核心 在基因组学和免疫学领域之间的接口项目，并获得了显着的 分析方面的专业知识：a）患有各种疾病的患者的血液; B）从 组织或细胞培养物; c）在体外暴露于广泛的先天刺激的血液;和d）培养于 抗原肽的存在。 将为下列项目提供支助：项目1（Banchereau）：分析在基因组中表达的基因， 体外产生的CD 8 + CTL;项目2（Palucka）：a）分析从接种疫苗的动物分离的T和B细胞; B）分析从humouse样品分离的人粘膜树突细胞（DC）;项目3（Sekaly）： 分析从接种疫苗的患者分离的CD 8 + CTL中表达的基因;项目4（Legrand）：a）分析 疫苗接种前后非人灵长类动物（NHP）血液中的抗原特异性免疫反应性 B）分析从NHP和人样品分离的人粘膜DC; 开发1（Eschawski）：将进行微阵列分析，以研究激活特性 不同的DC靶向疫苗。技术开发2（Pascual）：抗原特异性B 将从粘膜样品中分离细胞并用微阵列分析以建立它们的表型。