Core E

我们增加了核心E，以支持POI的新联合目标，这在即将到来的计划中都有描述 在项目3和更简短的项目1中。凯尔索博士，这个核心的PI，一直与 海恩斯、廖和哈里森，他是(与海恩斯、哈里森和开普勒)评论的合著者 描述(和命名)基于B细胞系的免疫原设计(Haynes，B.F.，Kelsoe，G.，Harison，S.C.， 以HLV-1为例研究疫苗开发中的B细胞系免疫原设计。自然界 生物技术10：423(2012)，PMC3512202)。这种新颖的单细胞B细胞培养系统将使我们能够 在易于处理的小鼠模型中，在可行的时间尺度上测试这一概念，它将产生应该有助于 生发中心反应和抗体亲和力成熟的更真实的模型(在人类以及 在小鼠中)在流感病毒免疫原的背景下。

We have added Core E, to support a new joint Aim of the POI, described in both in the plans for the coming year in Project 3 and more briefly in Project 1. Dr. Kelsoe, the Pi of this Core, has been working closely with Haynes, Liao, and Harrison, and he is a coauthor (with Haynes, Harrison, and Kepler) ofthe review that describes (and names) B-cell lineage based immunogen design (Haynes, B.F., Kelsoe, G., Harrison, S.C, Kepler, T.B. B-cell-lineage immunogen design in vaccine development with HlV-1 as a case study. Nature Biotechnology 10: 423 (2012), PMC3512202). The novel, single-cell, B-cell culture system will allow us to test this notion on a feasible timescale in a tractable mouse model, and it will yield data that should facilitate more realistic models of the germinal center reaction and antibody affinity maturation (in humans as well as in mice) in the context of an influenza virus immunogen.